ASCL1 Polyclonal Antibody

货号：PA5-77868

规格：100 µL

价格：5817

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant protein encompassing a sequence within

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:100-1:1000(ICC);1:100-1:1000(IF);1:100-1:1000(IHC(P));1:500-1:3000(WB)

产品详细信息Positive Control: U87-MG, SK-N-SH, IMR32, SK-N-AS, SH-SY5Y, SH-SY5Y nuclear extract, PC-12, Rat2Predicted Reactivity: Mouse (84%)Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. 靶标信息ASCL1 (also known as ASH1) is a basic helix-loop-helix transcription factor that is required for early development of the nervous system. Expressed in fetal brain, ASCL1 is essential for the proper development of autonomic neurons and for the survival of subsets of autonomic neurons. ASCL1 interaction with MEF-2A may regulate the expression of specific genes that are critical for the formation of distinct neuronal circuits within the central nervous system. The high level of ASCL1 expression in neuroendocrine tumors, such as medullary thyroid cancer, small cell lung cancer and lung cancer with neuroendocrine features may provide a useful marker for cancers with neuroendocrine features. Mapping to human chromosome 12, the ASCL1 gene contains a trinucleotide repeat region, making this locus a candidate for inherited disease.

数据：

ASCL1 Antibody (PA5-77868) in ICC Immunocytochemistry-Immunofluorescence analysis of ASCL1 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ASCL1 Polyclonal Antibody (Product # PA5-77868) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI. ASCL1 Antibody (PA5-77868) in IHC (P) Immunohistochemistry analysis of ASCL1 in paraffin-embedded rat brain. Sample was incubated with ASCL1 polyclonal antibody (Product # PA5-77868) using a dilution of 1:500. ASCL1 Antibody (PA5-77868) in WB Western blot was performed using Anti-ASCL1 Polyclonal Antibody (Product # PA5-77868) and 32 kDa band was observed corresponding to ASCL1 was observed across the cell lines tested along with an uncharacterized band (*) in Mouse pup brain. Modified Whole cell extracts (30ug lysate) (1% SDS) of Neural Stem cells (Lane 1), SH-SY5Y (Lane 2), U-87 MG (Lane 3), IMR-32 (Lane 4), Neuro-2A (Lane 5), tissue extracts of Mouse pup brain (Lane 6) and Rat pup brain (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

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