CD3e Monoclonal Antibody (eBio500A2 (500A2)), eBioscience™

货号：14-0033-81,14-0033-81,14-0033-85,14-0033-86

规格：50 µg,100 µg,500 µg,1 mg

价格：0,0,2176,4365

产品类型：流式抗体

品牌：eBioscience

抗原：CD3e

物种：小鼠

宿主：其它

抗体亚型：IgG

克隆号：eBio500A2 (500A2)

荧光染料：Purified

类型:	一抗	同型对照：	IgG
浓度：	0.5 mg/mL	用法：	0.5 µg/test（Flow）；20 µg/mL(IHC (F))

产品详细信息Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.Applications Reported: The eBio500A2 antibody has been reported for use in flow cytometric analysis and immunohistochemistry of frozen tissue. It has also been reported for use in in vitro activation of mouse T cells. (Please use Functional Grade purified eBio500A2 in functional assays).Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This eBio500A2 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 20 µmL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.仅用于科研。不用于诊断过程。

数据

CD3e Antibody (14-0033-82) in FlowStaining of BALB/c splenocytes with 0.25 µg of Golden Syrian Hamster IgG Isotype Control Purified (Product # 14-4914-81) (open histogram) or 0.25 µg of Anti-Mouse CD3e Purified (filled histogram) followed by Anti-Syrian Hamster IgG FITC (Product # 11-4211-85). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. The Journal of experimental medicineMast cells acquire MHCII from dendritic cells during skin inflammation."14-0033 was used in Flow cytometry/Cell sorting to identify an innate intercellular communication arming resident mast cells with key dendritic cell(DC) functions that might contribute to the acute defence potential during critical periods of migration-based DC absence."AuthorsDudeck J,Medyukhina A,Fröbel J,Svensson CM,Kotrba J,Gerlach M,Gradtke AC,Schröder B,Speier S,Figge MT,Dudeck AYear2017SpeciesMouse 2. Bio-protocolLymphocyte Isolation, Th17 Cell Differentiation, Activation, and Staining."14-0033 was used in Flow cytometry/Cell sorting to describe the tools, reagents and procdedure for a Th17 cell differentiation method."AuthorsKumar P,Kolls JKYear2016SpeciesMouse 3. NatureGlial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence."14003382 was used in flow cytometry to explain how glial cells impact ILC3-derived IL-22"AuthorsIbiza S,García-Cassani B,Ribeiro H,Carvalho T,Almeida L,Marques R,Misic AM,Bartow-McKenney C,Larson DM,Pavan WJ,Eberl G,Grice EA,Veiga-Fernandes HYear2016SpeciesMouse 4. The Journal of experimental medicineMicrobiota regulate the ability of lung dendritic cells to induce IgA class-switch recombination and generate protective gastrointestinal immune responses."14-0033 was used in Flow cytometry/Cell sorting to demonstrate the role of the microbiota in modulating the protective efficacy of intranasal vaccination through their effect on the IgA class-switching function of lung dendritic cells."AuthorsRuane D,Chorny A,Lee H,Faith J,Pandey G,Shan M,Simchoni N,Rahman A,Garg A,Weinstein EG,Oropallo M,Gaylord M,Ungaro R,Cunningham-Rundles C,Alexandropoulos K,Mucida D,Merad M,Cerutti A,Mehandru SYear2016SpeciesMouse 5. ImmunityT-box Transcription Factors Combine with the Cytokines TGF-β and IL-15 to Control Tissue-Resident Memory T Cell Fate."14-0033 was used in Flow cytometry/Cell sorting to indicate that the T-box TFs control the two cytokines, TGF-β and IL-15, which are pivotal for CD8(+)CD103(+) Trm cell development and survival."AuthorsMackay LK,Wynne-Jones E,Freestone D,Pellicci DG,Mielke LA,Newman DM,Braun A,Masson F,Kallies A,Belz GT,Carbone FR

技术参数

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