MBL Oligomer Human ELISA Kit ELISA试剂盒,MBL Oligomer Human ELISA Kit/人MBL低聚物ELISA试剂盒

2024-10-24

MBL Oligomer Human ELISA Kit/人MBL低聚物ELISA试剂盒

货号:KIT029

规格:96 tests

价格:8682

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

5 pg/mL

检测范围0-40 ng/mL

样本类型/体积

血清 10 μL

血浆 10 μL

操作时间

1 hr 20 min

测试时间

3 hr 15 min

Homogenous (no wash)

No

批内变异系数(CV)5.5%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 生物素化检测抗体

- SAV-HRP

- 洗涤浓缩液

- 显色剂

- 终止液

储存温度

2-8℃

Protein nameMBL2
Protein aliasesCollectin-1, mannan-binding lectin, Mannan-binding protein, Mannose-binding lectin, mannose-binding lectin (protein C) 2, soluble (opsonic defect), mannose-binding lectin 2, Mannose-binding protein C, MBP-C, MBP1

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCOLEC1, HSMBPC, MBL, MBL2, MBL2D, MBP, MBP-C, MBP1, MBPD
Gene ID(Human) 4153
Gene symbolMBL2
UniProt ID(Human) P11226
The Human Mannose Binding Lectin (Hu MBL-2) ELISA quantitates Hu MBL-2 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu MBL-2.人甘露糖结合凝集素(人 MBL-2)ELISA定量检测人血清或血浆中的MBL-2。该检测试剂盒将专门识别天然和重组人MBL-2。Principle of the method 方法原理The Human MBL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人MBL-2固相夹心ELISA(酶联免疫吸附测定)旨在测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品,标准品或质控品添加到这些孔中,并与固定的(捕获)抗体结合。通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样本中存在的目标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。有关验证的更多信息,请参见手册。Target information 靶标信息MBL2 is a calcium-dependent lectin involved in innate immune defense. It binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. It also binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. MBL2 may bind DNA.MBL2是涉及先天免疫防御的钙依赖性凝集素。它在不同微生物上结合甘露糖,岩藻糖和N-乙酰氨基葡萄糖,并激活凝集素补体途径。它也与晚期凋亡细胞,凋亡小泡和坏死细胞结合,但不与早期凋亡细胞结合,从而促进其被巨噬细胞摄取。 MBL2可以结合DNA。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

MBL Oligomer Human ELISA KitRepresentative standard curve for Human MBL ELISA

技术参数
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