Mouse IgG2a kappa Isotype Control (eBM2a), APC-eFluor 780, eBioscience™

货号：47-4724-81

规格：50 µg

价格：1406

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：eBM2a

荧光染料：APC-eFluor 780

抗体类型:	同型对照	同型对照：
浓度：	0.2 mg/mL	用法：	Assay-Dependent(Flow)； Assay-Dependent(Ctrl)

产品详细信息Description: This is a monoclonal mouse IgG2a antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This eBM2a antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells.Use isotype control at the same concentration as experimental antibody.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。

数据

Mouse IgG2a kappa Isotype Control (47-4724-81) in FlowStaining of human peripheral blood cells with CD19 Monoclonal Antibody, FITC (Product # 11-0199-42) and staining buffer (autofluorescence, left) or 0.25 µg of Mouse IgG2a kappa Isotype Control, APC-eFuor 780 (right). Total viable cells were used for analysis.

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技术参数

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