LSD1 Monoclonal Antibody (1B2E5)

货号：41-3300

规格：100 µg

价格：4809

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant protein derived from the internal regi

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：1B2E5

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.5 mg/mL	用法：	5-10 µg/mL(IHC(P));5 µg(IP);1-3 µg/mL(WB)

产品详细信息LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.

数据：

LSD1 Antibody (41-3300) in IHCImmmunohistochemistry analysis of breast carcinoma cells using mouse anti-LSD1 (Product # 41-3300) LSD1 Antibody (41-3300) in WBWestern blot analysis was performed on nuclear enriched extracts (30 µg lysate) of PC-3 (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3) and K-562 (Lane 4). The blots were probed with Anti-LSD-1 Mouse Monoclonal Antibody (Product # 41-3300, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 95 kDa band corresponding to LSD-1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with PierceTM Power Blotter System (Product # 22834). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). LSD1 Antibody (41-3300)Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with LSD1 siRNA and loss of signal was observed in Western Blot using Anti-LSD1 monoclonal Antibody (Product # 41-3300).Knockdown validation info.

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