SIRT6 Monoclonal Antibody (2G1H1)

货号：MA1-214

规格：100 µg

价格：4553

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Purified recombinant fragment of human SIRT6 (AA:

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：2G1H1

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:100-1:200(ICC)；1:100-1:200（IF）；1:1000（WB）

产品详细信息MA1-214 SIRT6 antibody detects SIRT6 by western blot and immunofluroescence.MA1-214 SIRT6 antibody detects SIRT6 by western blot at ~42kDa.靶标信息Sirtuins regulate epigenetic gene silencing. SIRT6 (Mono-ADP-ribosyltransferase sirtuin-6), a mouse Sir2 homolog, is a chromatin-associated nuclear ADP-ribosyltransferase protein. It appears to be involved in DNA repair and may also play a role in human aging.

数据：

SIRT6 Antibody (MA1-214) in IFImmunofluorescent analysis of SIRT6 (green) in MCF7 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at -20c, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a SIRT6 mouse monoclonal antibody (Product # MA1-214) at a dilution of 1:100 in blocking buffer overnight at 4°C, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32731) at a dilution of 1:1000 for at least 30 minutes at a room temperature in the dark (green). F-actin (red) was stained with Dylight 554 Phalloidin. Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification. SIRT6 Antibody (MA1-214) in WBWestern blot analysis of SIRT6 was performed by loading 20 µg of indicated whole cell lysates and 7 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. SIRT6 was detected at 42 kDa using a SIRT6 mouse monoclonal antibody (Product # MA1-214, upper panel) at a dilution of 1:1000 in blocking buffer overnight at 4°C on a rocking platform, followed by a Goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 31430) at a dilution of 1:20000 for at least 30 minutes at room temperature. Beta-Tubulin was detected using mouse monoclonal antibody (Product # MA5-16308, lower panel), followed by a Goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 31430) at a dilution of 1:20000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076).

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