SIRT1 Monoclonal Antibody (OTI5B2)

货号：MA5-27217

规格：100 µL

价格：5956

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Human recombinant protein fragment corresponding t

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：OTI5B2

荧光染料：Alexa Fluor 488

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:100(ICC);1:100(IF);1:2000(IHC(P));1:500-1:2000(WB)

靶标信息NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, repsonse to DNA damage, metabolism, apoptosis, and autophagy. SIRT1 can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histone and DNA, leading to transcriptional repression. It is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT).

数据：

SIRT1 Antibody (MA5-27217) in ICCImmunofluorescence analysis of SIRT1 was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SIRT1 Monoclonal Antibody (Product # MA5-27217) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. SIRT1 Antibody (MA5-27217) in IHC (P)Immunohistochemistry was performed on paraffin-embedded carcinoma of human lung tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a SIRT1 monoclonal antibody (Product # MA5-27217) at a dilution of 1:2000. SIRT1 Antibody (MA5-27217) in WBWestern blot was performed using Anti-SIRT1 Monoclonal Antibody (OTI5B2) (Product # MA5-27217) and a 110 kDa band corresponding to SIRT1 was observed in all cell and tissue lysates tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of MCF7 (Lane 1), HEK-293 (Lane 2), NTERA-2 cl.D1 (Lane 3), Mouse Testis (Lane 4), Mouse Ovary (Lane 5) and Rat Ovary (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band (*) was also observed at ~40 kDa.

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