Survivin Polyclonal Antibody

货号：PA1-16836

规格：100 µL

价格：5548

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant human survivin, full-length.

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:10-1:500（ChIP）;1:50-1:250(ICC);1:50-1:250(IF);1:50-1:500(IHC(P));1:10-1:500(IP);1:1000(WB)

产品详细信息For IHC, prior antigen retrieval (pressure cooking) is recommended for cytoplasmic and nuclear detection of Survivin.Suggested positive control: Hela whole cell extract, antigen standard for BIRC5 (transient overexpression lysate).靶标信息Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin's gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.

数据：

Survivin Antibody (PA1-16836) in IFTelophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection usingProduct # PA1-16836. Survivin Antibody (PA1-16836) in IHCThe top photo is a no primary antibody control stain and the bottom photo is anti-survivin staining of melanoma. Survivin Antibody (PA1-16836) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of HT29 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), SK-BR-3 (Lane 4) and SK-BR-3 treated with LY294002 (10uM for 24 hours) (Lane 5). The blot was probed with Anti-Survivin Polyclonal Antibody (Product # PA1-16836, 1µg/mL dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Survivin was observed in all the cell lines tested and was observed to reduce in SK-BR-3 cell line upon treatment with LY294002.

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