Survivin Monoclonal Antibody (8E2)

货号：MA5-11680

规格：500 µL

价格：5931

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Full length recombinant human survivin protein

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：8E2

荧光染料：Alexa Fluor 488

类型:	一抗	同型对照：
浓度：	0.2mg/mL	用法：	1:10-1:200(ICC);1:10-1:200(IF);1:10-1:100(IHC(P))

产品详细信息MA5-11680 targets Survivin in ICC/IF and IHC (P) applications and shows reactivity with Human and Rat samples.The MA5-11680 immunogen is full length recombinant human survivin protein.靶标信息Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin's gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.

数据：

Survivin Antibody (MA5-11680) in IFImmunofluorescent analysis of Survivin (green) showing staining in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Survivin monoclonal antibody (Product # MA5-11680) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x. Survivin Antibody (MA5-11680) in IHC (P)Immunohistochemistry analysis of Survivin showing positive staining in the cytoplasm and nucleus of paraffin-treated Human bladder carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Survivin monoclonal antibody (Product # MA5-11680) diluted by 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

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