BRD4 Polyclonal Antibody

货号：PA5-41550

规格：100 µL

价格：4805

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide directed towards the C-terminal

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.5mg/mL	用法：	0.5 µg(ChIP);4 µg/mL（ICC）；4 µg/mL(IF);0.2-1 µg/mL(WB）

产品详细信息Peptide sequence: EIEIDFETLK PSTLRELERY VTSCLRKKRK PQAEKVDVIA GSSKMKGFSSSequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%靶标信息BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycleby interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.

数据：

BRD4 Antibody (PA5-41550) in IFImmunofluorescence analysis of HeLa cells using an anti-BRD4 polyclonal antibody (Product # PA5-41550). Primary Antibody Dilution: 4 µg/mL; Secondary Antibody: Anti-rabbit Alexa 546; Secondary Antibody Dilution: 2 µg/mL. BRD4 Antibody (PA5-41550) in WBWestern blot analysis of BRD4. 50 µg total protein lysate from human embryonic kidney HEK293T cells (lane 2) and mouse myoblast C2C12 cells (lane 3) in 1X NuPAGE® LDS Sample Buffer (Product # NP0007) + 1mM DTT loaded onto a NuPAGE™ Novex™ 4-12% Bis-Tris protein gel (Product # NP0321PK2). Molecular weight marker loaded for size reference (lane 1). Gel run in 1X NuPAGE® MOPS SDS Running Buffer (Product # NP0001) at room temperature and then transferred to a methanol-activated Novex™ PVDF membrane (Product # LC2002) using a Invitrogen Mini Blot Module (Product # B1000) in 1X NuPAGE® Transfer Buffer (Product # NP0006) at 20V for 2 hours at room temperature. Membrane was rinsed in 1X TBS-T and incubated in blocking solution (5% milk in 1X TBS-T) for 1 hour at room temperature on a rocking platform. HDAC1 detection was performed by incubating blot with rabbit polyclonal anti-BRD4 antibody (Product # PA5-41550; 0.9 mg/mL) diluted at 1:1000 in blocking solution for 1 hour at room temperature on a rocking platform. Primary antibody was washed 10 times with 1X TBS-T over the course of 1 hour at room temperature on a rocking platform. Secondary antibody binding was performed by incubation with goat anti-rabbit IgG-HRP secondary diluted at 1:5000 in blocking solution for 1 hour at room temperature on a rocking platform. Following washes (as above), chemiluminescent imaging was performed. Data courtesy of Dr. Raj Jain's laboratory.

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