EED Polyclonal Antibody

货号：PA5-34430

规格：100 µg

价格：6049

产品类型：标签抗体

品牌：Thermo Fisher

抗原： An 18 amino acid synthetic peptide near the amino

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	2.5 µg/10^6 cells（ChIP）;20 µg/mL（ICC）；20 µg/mL(IF);2 µg/mL(WB）

产品详细信息A suggested positive control is human heart tissue lysate.PA5-34430 can be used with blocking peptide PEP-1472.靶标信息The EED protein (WAIT-1 or WD protein associated with integrin cytoplasmic tails-1), also called embryonic ectoderm development, is a member of the superfamily of WD-40 repeat proteins and widely conserved polycomb group (PcG) family of proteins. The polycomb group (PcG) is a large and evolutionarily conserved set of genes whose products act in multimeric complexes to modify histones, which are then thought to cause stable and heritable states of transcriptional repression. EED shuttles betweenthe nucleus and the plasma membrane and can interact with the cytoplasmic tail of integrin Beta-7 subunit. EED exerted an antiviral activity at the late stage of HIV-1 replication.

数据：

EED Antibody (PA5-34430) in IFImmunofluorescence analysis of EED was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EED Rabbit Polyclonal Antibody (Product # PA5-34430) at 20 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.. EED Antibody (PA5-34430) in WBWestern blot was performed using Anti-EED Polyclonal Antibody (Product # PA5-34430) and a 45kDa band corresponding to human EED isoform and 56kDa band corresponding to mouse EED isoform was observed across cell lines and tissues tested. In addition to this there was an increase in the expression of EED proteins upon SAHA treatment in A549 cells. Whole cell extracts (30 µg lysate) of A549 (Lane 1), A549 treated with SAHA (2uM for 72hr) (Lane 2), tissue extracts (30 µg lysate) of Rat Kidney (Lane 3), Mouse Lung (Lane 4), Mouse Spleen (Lane 5) and Mouse Pancreas (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

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