PRMT1 Monoclonal Antibody (GT779)

货号：MA5-27756

规格：100 µL

价格：5817

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Carrier-protein conjugated synthetic peptide encom

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：GT779

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:100-1:1000(ICC) ;1:100-1:1000(IF);1:100-1:1000(IHC(P));1:500-1:1,000(WB)

产品详细信息Positive Control: Neuro2A, GL261, NIH-3T3, Raw264.7, C2C12, 293T, A431, HeLa, HepG2Predicted Reactivity: Rat (100%), Xenopus laevis (100%), Bovine (100%)Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. 靶标信息PRMT1 is a member of the protein arginine N-methyltransferase (PRMT) family. Arginine methylation is an irreversible post translational modification which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Increased expression of PRMT1 may play a role in various types of cancer.

数据：

PRMT1 Antibody (MA5-27756) in IF Immunofluorescence analysis of PRMT1 was performed using HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PRMT1 Mouse Monoclonal Antibody (Product # MA5-27756) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green) in HCT 116 cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HCT 116 cells showing a predominantly nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. PRMT1 Antibody (MA5-27756) in IHC (P)Immunohistochemistry (Paraffin) analysis of PRMT1 was performed in paraffin-embedded human ovarian cancer tissue using PRMT1 Monoclonal Antibody (GT779) (Product # MA5-27756) at a dilution of 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

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