Ovine IFN-gamma ELISA Kit ELISA试剂盒,Ovine IFN-gamma ELISA Kit/绵羊干扰素-γ ELISA试剂盒

2024-10-24

Ovine IFN-gamma ELISA Kit/绵羊干扰素-γ ELISA试剂盒

货号:ESH1RB,ESH1RBX5

规格:96 Tests,5X96 Tests

价格:6641,29884

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:山羊

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

5 pg/mL

检测范围5-1,000 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

5 x 96 Tests

组份

- 预包被96孔板

- 标准品

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameIFN gamma
Protein aliasescytokine, gamma-interferon, IFN-gamma, IFN-gamma precursor, Interferon gamma

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesIFN-gamma, IFNG
Gene ID(Sheep) 443396
Gene symbolIFNG
UniProt ID(Sheep) P17773
Ovine IFN-gamma quantitates ovine IFN-gamma in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant ovine IFN-gamma.绵羊IFN-γ定量测定血清,血浆,上清液中的绵羊IFN-γ。该检测试剂盒将只识别天然和重组绵羊IFN-γ。Target information 靶标信息IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.IFNγ(干扰素γ,II型干扰素)是一种巨噬细胞活化因子,是一种免疫干扰素,主要由T淋巴细胞和自然杀伤细胞响应抗原,促分裂原,葡萄球菌肠毒素B,植物血凝素和其他细胞因子而产生。 IFNγ是由两个146个氨基酸亚基组成的二聚体蛋白。 IFNγ是一种糖蛋白,功能上以约45 kDa的同型二聚体形式存在。在SDS-PAGE上,由于差异糖基化,IFNγ出现了25、20和15.5 kDa的较小条带。 IFNγ同二聚体的生物活性具有高度的物种特异性。人类IFNγ与小鼠无交叉反应。 IFNγ功能包括:抗病毒活性,肿瘤抗增殖活性,I类和II类MHC的诱导,巨噬细胞活化以及B淋巴细胞增强的免疫球蛋白分泌。 IFNγ参与细胞因子的调节,并与其他细胞因子协同作用。 IFNγ的激活是通过结合IFNγI和II并激活JAK-STAT途径进行的。 IFNγ与IFNα或IFNβ没有任何同源性,但是人IFNγ与小鼠IFNγ具有约40%的序列同源性。 IFNγ被IL2,FGF碱性蛋白,EGF上调,而被维生素D3或DMN下调。 IFNγ基因突变与再生障碍性贫血有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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