Ovine IL-12 p70 ELISA Kit ELISA试剂盒,Ovine IL-12 p70 ELISA Kit/绵羊IL-12 p70 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Ovine IL-12 p70 ELISA Kit ELISA试剂盒,Ovine IL-12 p70 ELISA Kit/绵羊IL-12 p70 ELISA试剂盒

2024-10-24

Ovine IL-12 p70 ELISA Kit/绵羊IL-12 p70 ELISA试剂盒

货号：ESH2RB,ESH2RBX5

规格：96 Tests,5X96 Tests

价格：6641,29884

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：山羊

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	2.5 pg/mL
检测范围	2.5-500 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	4 hr 45 min
Homogenous (no wash)	No
批间变异系数（CV）	<12%
批内变异系数（CV）	<10%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	5 x 96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-12 p70
Protein aliases	CLMF p35, CLMF p40, cytokine, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12, IL-12 subunit p35, IL-12 subunit p40, Il-12, IL-12A, IL-12B, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35), interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), interleukin-12 p40 subunit, Interleukin-12 subunit alpha, Interleukin-12 subunit beta
物种（Tested）	羊
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	IL-12, IL12, IL12A, IL12B, IL12P35
Gene ID	(Sheep) 443064,(Sheep) 443472
Gene symbol	IL12A, IL12B
UniProt ID	(Sheep) Q9TU27,(Sheep) P68220

Ovine IL-12 p70 quantitates ovine IL-12 p70 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant ovine IL-12 p70.绵羊IL-12 p70定量测定血清，血浆，上清液中的绵羊IL-12 p70。该检测试剂盒将只识别天然和重组绵羊IL-12 p70。Target information 靶标信息Interleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. IL-12 p70 is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Human and mouse IL-12 p40 share about 70% amino acid sequence homology.白介素-12（IL-12）是一种异源二聚体70 kDa细胞因子，由两条共价连接的糖基化链组成，分子量分别为40kD（p40）和35-kD（p35）。 IL-12主要由单核细胞，巨噬细胞和树突状细胞响应细菌产品（如脂多糖（LPS）），细胞内病原体或与活化T细胞相互作用而产生。最初发现IL-12是因为它具有诱导干扰素-γ（IFN-g）产生，细胞增殖以及由自然杀伤细胞和T细胞介导的细胞毒性的能力。研究表明，IL-12在Th1反应的发展中也起着关键作用，导致IFN-g和IL-2的产生。这些细胞因子可进而促进T细胞反应和巨噬细胞活化。使用双启动子表达系统，在杆状病毒感染的昆虫细胞中产生重组小鼠IL-12 p70，作为前体p35和p40亚基的真正异源二聚体。 IL-12 p70与其他可用形式的蛋白质不同，因为它表达为真正的异二聚体，而不是单链的假异二聚体，其中亚基通过人工接头连接。淋巴细胞对此细胞因子的反应是由转录蛋白STAT4的激活剂介导的。发现一氧化氮合酶2A（NOS2A / NOS2）是先天免疫中该细胞因子信号传导过程所必需的。人和小鼠IL-12 p40共有约70％的氨基酸序列同源性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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Ovine IL-12 p70 ELISA Kit ELISA试剂盒,Ovine IL-12 p70 ELISA Kit/绵羊IL-12 p70 ELISA试剂盒

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