20-Hydroxyecdysone ELISA Kit ELISA试剂盒,20-Hydroxyecdysone ELISA Kit/20-羟基蜕皮激素ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

20-Hydroxyecdysone ELISA Kit ELISA试剂盒,20-Hydroxyecdysone ELISA Kit/20-羟基蜕皮激素ELISA试剂盒

2024-10-24

20-Hydroxyecdysone ELISA Kit/20-羟基蜕皮激素ELISA试剂盒

货号：EIAHYD,EIAHYDX10

规格：96 tests,10X96 tests

价格：6641,57271

产品类型：ELISA检测

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	197 pg/mL
检测范围	102.4-25,000 pg/mL
样本类型/体积	血清 50 µL 血浆 50 µL 粪便提取物 50 µL 尿液 12.5 µL 上清液 50 µL
操作时间	45 min
测试时间	2 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	9%
批内变异系数（CV）	10%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 抗-lgG包被96孔板 - 标准品 - 测定稀释浓缩液 - 捕获抗体 - HRP结合抗原 - 洗涤浓缩液 - TMB底物 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	20-Hydroxyecdysone
物种（Tested）	人
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The 20-Hydroxyecdysone Competitive ELISA quantitates 20-Hydroxyecdysone (20E) in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.|Principle of the method: The 20-Hydroxyecdysone Competitive ELISA research-use-only kit is designed to quantitatively measure 20-Hydroxyecdysone independent of species. A 20-Hydroxyecdysone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit 20-Hydroxyecdysone antibodies and 20-Hydroxyecdysone-peroxidase conjugate is added to the wells. The 20-Hydroxyecdysone-peroxidase conjugate and any 20-Hydroxyecdysone in the sample will compete to bind to the anti-rabbit 20-Hydroxyecdysone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound 20-Hydroxyecdysone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of 20-Hydroxyecdysone present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.20羟蜕皮激素竞争性ELISA定量分析血清，血浆，尿液，唾液，干燥的粪便提取物或细胞培养基中的20羟蜕皮激素（20E）。定量测量20-羟基蜕皮激素，与物种无关。提供了20-羟基蜕皮激素标准品以生成测定的标准曲线，并从用户生成的标准曲线中读取所有样品。将标准品或稀释的样品吸移到涂有抗兔IgG抗体的透明微量滴定板中。将抗兔20-羟基蜕皮激素抗体和20-羟基蜕皮激素-过氧化物酶结合物添加到孔中。样品中的20-羟基蜕皮激素-过氧化物酶结合物和任何20-羟基蜕皮激素将竞争结合抗兔20-羟基蜕皮激素抗体。孵育后，洗涤板并加入底物。底物与结合的20-羟基蜕皮激素-过氧化物酶结合物反应。孵育30分钟后，停止反应，并在微量滴定板读数器中于450 nm处检测所产生颜色的强度。产生的颜色的强度与样品中20-羟基蜕皮激素的含量成反比。|严格的验证：每个ELISA试剂盒的生产批次均经过质量测试，包括敏感性，特异性，精密度和批次之间的标准。一致性。有关验证的更多信息，请参见手册。Target information 靶标信息20-Hydroxyecdysone is the most widely occurring ecdysteroid in both plant and animal species. It controls cell death during metamorphosis of Drosophila melanogaster.20-羟基蜕皮激素是动植物中最广泛存在的蜕皮类固醇。它控制果蝇变态过程中的细胞死亡。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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20-Hydroxyecdysone ELISA Kit ELISA试剂盒,20-Hydroxyecdysone ELISA Kit/20-羟基蜕皮激素ELISA试剂盒

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