EC0112,DH5α Competent Cells/DH5α 感受态细胞-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

EC0112,DH5α Competent Cells/DH5α 感受态细胞

2024-10-24

DH5α Competent Cells/DH5α 感受态细胞

货号：EC0112

规格：10 x 100 µL

价格：1205

产品类型：感受态细胞

品牌：Thermo Fisher

Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal. RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA.• High transformation efficiency: >1x109cfu/µg pUC19 DNA• Suitable for routine and high-throughput cloning applications• Genetic markers that allow for blue/white colony screening• Convenient two reactions per vial packaging• S.O.C. Outgrowth medium includedApplicationsDH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:• Efficient DNA cloning derived from PCR, cDNA-generation reactions• recA1 marker facilitates working with difficult to transform DNA• Ideal for generation of cDNA libraries using plasmid-derived vectors• Site-directed mutagenesisGenotypeF– φ80lacZΔ M15 Δ (lacZYA-argF)U169 recA1 endA1 hsdR17(rK– mK+)phoA supE44λ-thi–1 gyrA96 relA1

优点：

- High transformation efficiency: >1x109cfu/µg pUC19 DNA- Suitable for routine and high-throughput cloning applications- Genetic markers that allow for blue/white colony screening- Convenient two reactions per vial packaging- S.O.C. Outgrowth medium included

数据：

图1.Transformation efficiency of DH5α Competent CellsThermo Scientific DH5α competent cells provide high transformation efficiency. The transformation efficiencies of DH5α competent cells and NEB 5-alpha competent E. coli were compared using pUC19 DNA and following the manufacturers’ recommended protocols. The transformation efficiency specification for DH5α competent cells is 1.0 x 109CFU/µg DNA (represented by the red dashed line). The values shown are the averages of five transformations.

组成成分：

▪DH5α Competent Cells ▪pUC19 DNA (10 pg/µL) ▪S.O.C. Medium

EC0112,DH5α Competent Cells/DH5α 感受态细胞

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