Thermo Fisher 内切酶GsuI ER0461,GsuI (BpmI、Bco35I、Bsp22I) (5 U/µL)/内切酶-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo Fisher 内切酶GsuI ER0461,GsuI (BpmI、Bco35I、Bsp22I) (5 U/µL)/内切酶

2024-10-24

GsuI (BpmI、Bco35I、Bsp22I) (5 U/µL)/内切酶

货号：ER0461,ER0462

规格：100 units,500 units

价格：1063,4722

产品类型：内切酶

品牌：Thermo Fisher

Thermo Scientific GsuI (BpmI) restriction enzyme recognizes CTGGAG(16/14)^ sites and cuts best at 30°C in B buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion. Isoschizomers: BpmI.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:Incubation at 37°C results in 70% activity.GsuI requires only Mg2+ for its activity, but is stimulated by S-adenosylmethionine. 0.01 mM S-adenosylmethionine gives a 2-fold increase in activity.GsuI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.GsuI is blocked by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163.For methylation sensitivity, refer to product specifications.Thermo Scientific GsuI (BpmI) 限制性内切酶可识别 CTGGAG(16/14)^ 位点，于 30°C 下在 B 缓冲液中的切割效果最佳。有关本限制性内切酶及其他限制性内切酶的酶活性、双酶切条件和热灭活的表格，请参见限制性内切酶的反应条件。备注：另外提供用于快速 DNA 酶切的FastDigest 酶。同裂酶：BpmI。Thermo Scientific 常规限制性核酸内切酶是高质量限制性内切酶的大量汇集物，经过优化以便在五缓冲液系统的一种缓冲液中发挥作用。此外还提供通用 Tango 缓冲液，便于进行双酶切。所有酶在推荐的缓冲液和反应条件下均表现出 100% 活性。为确保一致的性能，Thermo Scientific 限制性内切酶反应缓冲液包含预混合 BSA，其可增强多种酶的稳定性，并与 DNA 制剂中可能存在的污染物相结合。注：在 37°C 下孵育时具有 70% 活性。GsuI 仅需要 Mg2+ 即可具有活性，但会受 S-腺苷蛋氨酸的刺激。0.01 mM S-腺苷蛋氨酸使活性增加至 2 倍。GsuI 可能与切割的 DNA 连接在一起。这会导致电泳中出现 DNA 条带漂移。为避免非典型 DNA 带型，使用 6X DNA 上样染料与 SDS 溶液进行样品制备，或在电泳前在存在 SDS 的条件下加热酶切的 DNA。GsuI 会被重叠 dcm 甲基化阻断。为避免 dcm 甲基化，使用 dam-、dcm-菌株，如 GM2163。对 dam 甲基化不敏感、对 dcm 甲基化敏感，对 CpG 甲基化不敏感

优点：

- Superior quality—stringent quality control and industry leading manufacturing process- Convenient color-coded Five Buffer System- Includes universal Tango buffer for double-digestions- BSA premixed in reaction buffers- Wide selection of restriction endonuclease specificities- 优异的质量—严格的质量控制和业内领先的生产流程- 使用颜色标记的五大便捷缓冲系统- 包含适于双酶切的通用型Tango缓冲液- 反应缓冲液中预先混入BSA- 种类齐全，特异性限制性内切酶的广泛选择

数据：

技术参数

产品优点 1. 卓越的质量—严格的质量控制和行业领先的生产工艺 2. 便利的颜色编码的五缓冲液系统 3. 包括用于双酶切的通用型 Tango 缓冲液 4. 在反应缓冲液中预混合 BSA 5. 多种特异性限制性核酸内切酶可供选择

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Thermo Fisher 内切酶GsuI ER0461,GsuI (BpmI、Bco35I、Bsp22I) (5 U/µL)/内切酶

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