Thermo Fisher SalI内切酶 ER0641 ,SalI (10 U/µL)/SalI内切酶-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo Fisher SalI内切酶 ER0641 ,SalI (10 U/µL)/SalI内切酶

2024-10-24

SalI (10 U/µL)/SalI内切酶

货号：ER0641,ER0642,ER0645

规格：1500 units,5 x 1500 units,2000 units

价格：333,1331,398

产品类型：内切酶

品牌：Thermo Fisher

Thermo Scientific SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:Incubation at 25°C results in 50-75% activity. Low salt, high glycerol (> 5%) concentrations, pH> 8.0 or a large excess of enzyme may result in star activity. Supercoiled forms of pBR322 and pUC require 10-fold overdigestion with SalI to achieve complete digestion. For methylation sensitivity, refer to product specifications.Thermo Scientific SalI 限制性内切酶可识别 G^TCGAC 位点，于37°C 下在O 缓冲液中的切割效果最佳。有关该酶和其他限制性内切酶的酶活性、双重酶切条件和热灭活的表格，请参见限制性内切酶的反应条件。备注：另外提供用于快速 DNA 酶切的FastDigest 酶。Thermo Scientific 常规限制性核酸内切酶是高质量限制性内切酶的大量汇集物，经过优化以便在五缓冲液系统的一种缓冲液中发挥作用。此外还提供通用 Tango 缓冲液，便于进行双酶切。所有酶在推荐的缓冲液和反应条件下均表现出 100% 活性。为确保一致的性能，Thermo Scientific 限制性内切酶反应缓冲液包含预混合 BSA，其可增强多种酶的稳定性，并与 DNA 制剂中可能存在的污染物相结合。备注：在 25°C 下孵育可实现 50-75% 活性。低盐、高甘油 (>5%) 浓度、pH 值 > 8.0 或酶过量可能导致星活性。为实现完全酶切，pBR322 和 pUC 的超螺旋形态需要 10 倍 SalI 过度酶切。对 dam 甲基化不敏感、对 dcm 甲基化不敏感、对 CpG 甲基化敏感。

优点：

- Superior quality—stringent quality control and industry leading manufacturing process- Convenient color-coded Five Buffer System- Includes universal Tango buffer for double-digestions- BSA premixed in reaction buffers- Wide selection of restriction endonuclease specificities- 卓越的质量—严格的质量控制和行业领先的生产工艺- 便利的颜色编码的五缓冲液系统- 包括用于双酶切的通用型 Tango 缓冲液- 在反应缓冲液中预混合 BSA- 多种限制性核酸内切酶特异性可供选择

数据：

技术参数

产品优点 1. 卓越的质量—严格的质量控制和行业领先的生产工艺 2. 便利的颜色编码的五缓冲液系统 3. 包括用于双酶切的通用型 Tango 缓冲液 4. 在反应缓冲液中预混合 BSA 5. 多种限制性核酸内切酶特异性可供选择

产品应用 •分子克隆 •限制位点作图 •基因分型 •Southern 印迹 •限制性片段长度多态性 (RFLP) •SNP

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-47872.htm

特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系删除，本平台不提供信息存储服务。

Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.

产品询价需求提交

Thermo Fisher SalI内切酶 ER0641 ,SalI (10 U/µL)/SalI内切酶

相关推荐