DNA内切酶,thermo,SmaI, HC (50 U/µL)

2024-10-24

SmaI, HC (50 U/µL)

货号:ER0663

规格:6000 units

价格:1646

产品类型:内切酶

品牌:Thermo Fisher

Thermo Scientific SmaI restriction enzyme recognizes CCC^GGG sites and cuts best at 30°C in Tango buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Isoschizomers: TspMI, XmaCI; neoschizomer: XmaI.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Thermo Scientific SmaI限制性内切酶可识别CCC ^ GGG位点,并在Tango缓冲液中于30°C切割效果最佳。有关此酶及其他限制酶的酶活性,双重消化条件和热灭活条件,请参见限制酶的反应条件表格。 注意:也可以作为FastDigest酶用于快速DNA消化。同分异构体:TspMI, XmaCI;异裂酶:XmaI。Thermo Scientific常规限制性内切核酸酶是大量高质量的限制性内切酶,经过优化可在五种缓冲液系统的一种缓冲液中工作。 此外,通用的Tango缓冲液为双消化提供了便利。 在推荐的缓冲液和反应条件下,所有酶均具有100%的活性。 为确保性能稳定,Thermo Scientific限制性内切酶反应缓冲液包含预混合的BSA,可增强许多酶的稳定性并结合DNA制备物中可能存在的污染物。

优点:
• Superior quality—stringent quality control and industry leading manufacturing process• Convenient color-coded Five Buffer System• Includes universal Tango buffer for double-digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificities• 优异的质量—严格的质量控制和业内领先的生产流程• 使用颜色标记的五大便捷缓冲系统• 包含适于双酶切的通用型Tango缓冲液• 反应缓冲液中预先混入BSA• 种类齐全,特异性限制性内切酶的广泛选择
数据:

技术参数

产品优点 • Superior quality—stringent quality control and industry leading manufacturing process • Convenient color-coded Five Buffer System • Includes universal Tango buffer for double-digestions • BSA premixed in reaction buffers • Wide selection of restriction endonuclease specificities • 优异的质量—严格的质量控制和业内领先的生产流程 • 使用颜色标记的五大便捷缓冲系统 • 包含适于双酶切的通用型Tango缓冲液 • 反应缓冲液中预先混入BSA • 种类齐全,特异性限制性内切酶的广泛选择

产品应用 • Molecular cloning • Restriction site mapping • Genotyping • Southern blotting • Restriction fragment length polymorphism (RFLP) • SNP Note: For methylation sensitivity, refer to product specifications. •分子克隆 •限制性位点比对 •基因分型 •Southern印迹 •限制性片段长度多态性(RFLP) •SNP(单核苷酸多态性) 注意:有关甲基化敏感性的信息,请参阅产品说明。

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