Thermo Fisher DNA内切酶NheI ER0971,NheI （AsuNHI、BmtI）(10 U/µL)/内切酶-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo Fisher DNA内切酶NheI ER0971,NheI （AsuNHI、BmtI）(10 U/µL)/内切酶

2024-10-24

NheI （AsuNHI、BmtI）(10 U/µL)/内切酶

货号：ER0971,ER0972,ER0975

规格：500 units,2500 units,1000 units

价格：520,2074,896

产品类型：内切酶

品牌：Thermo Fisher

Thermo Scientific NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (isoschizomers: AsuNHI, BmtI).SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:Low salt, high glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity. NheI is inhibited by salt concentrations above 100mM. Supercoiled plasmids may require up to 10-fold more NheI for complete digestion than linear DNAs (e.g. 10 units are required to cleave 1µg of pBR322 DNA.) For methylation sensitivity, refer to product specifications.Thermo Scientific NheI 限制性内切酶可识别 G^CTAGC 位点，于 37°C 下在 Tango 缓冲液中的切割效果最佳（同裂酶：AsuNHI、BmtI）。，有关该酶和其他限制性内切酶的酶活性、双重酶切条件和热灭活的表格，请参见限制性内切酶的反应条件。备注：另外提供用于快速 DNA 酶切的FastDigest 酶。Thermo Scientific 常规限制性核酸内切酶是高质量限制性内切酶的大量汇集物，经过优化以便在五缓冲液系统的一种缓冲液中发挥作用。此外还提供通用 Tango 缓冲液，便于进行双酶切。所有酶在推荐的缓冲液和反应条件下均表现出 100% 活性。为确保一致的性能，Thermo Scientific 限制性内切酶反应缓冲液包含预混合 BSA，其可增强多种酶的稳定性，并与 DNA 制剂中可能存在的污染物相结合。对 dam 甲基化不敏感、对 dcm 甲基化不敏感、对 CpG 甲基化敏感。备注：低盐、高甘油 (>5%) 浓度、pH 值 > 8.0 或酶过量可能导致星活性。高于 100mM 的盐浓度会抑制 NheI。为实现完全酶切，超螺旋质粒需要的 NheI 可能是线性 DNA 所需量的 10 倍（例如，需要 10 个单位来切割 1µg pBR322 DNA。

优点：

- Superior quality—stringent quality control and industry leading manufacturing process- Convenient color-coded Five Buffer System- Includes universal Tango buffer for double-digestions- BSA premixed in reaction buffers- Wide selection of restriction endonuclease specificities- 卓越的质量—严格的质量控制和业内领先的生产流程- 使用颜色标记的五大便捷缓冲系统- 包含适于双酶切的通用型Tango缓冲液- 反应缓冲液中预先混入BSA- 种类齐全，特异性限制性内切酶的广泛选择

数据：

技术参数

产品优点 1. 卓越的质量—严格的质量控制和行业领先的生产工艺 2. 便利的颜色编码的五缓冲液系统 3. 包括用于双酶切的通用型 Tango 缓冲液 4. 在反应缓冲液中预混合 BSA 5. 多种限制性核酸内切酶特异性可供选择

产品应用 •分子克隆 •限制位点作图 •基因分型 •Southern 印迹 •限制性片段长度多态性 (RFLP) •SNP

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Thermo Fisher DNA内切酶NheI ER0971,NheI （AsuNHI、BmtI）(10 U/µL)/内切酶

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