Thermo Template Generation System II Kit,Template Generation System II Kit-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo Template Generation System II Kit,Template Generation System II Kit

2024-10-24

Template Generation System II Kit

货号：F702

规格：20 reactions

价格：2670

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

描述Thermo Scientific transposon technology is the first technology that allows scientist to insert constructed linear DNA fragment into circular or linear DNA target randomly and very efficiently. The Thermo Scientific Template Generation System II Kit (TGS II Kit) is based on the transposition reaction of the bacteriophage Mu. This system has been simplified to work in vitro, and an artificial Mu transposon, designated as the Entranceposon, has been constructed. The reaction is catalyzed by a single enzyme, MuA Transposase.TGS II Kit provides the tools for inserting the Entranceposon into foreign DNA at random locations. Insertion of an Entranceposon into unknown target DNA provides primer binding sites for different applications. A simple and fast in vitro transposition reaction takes place in a single reaction tube.Features:• Provides templates for DNA sequencing more simply and with less hands-on time than any other method• Thousands of ready-to-sequence templates from a single transposition reaction: Enough to sequence even large DNA clones (e.g. BAC clones) without fragmentation or subcloning• Simple mapping of the Entranceposon insertions by colony-PCR or restriction enzyme digestion enables directed sequencing. Fewer sequencing reactions are required to complete a sequence than with the 'shotgun' approach• Universal primers: The sequencing and mapping primers are included in the kit, no need for custom primers.• Bidirectional sequencing: A single template clone can be used for sequencing the flanking DNA on both sides of the Entranceposon insertion• Three different antibiotic resistance genes are available in each TGS II Kit• All components needed as well as detailed instruction manual to perform the reactions are included in the kit. TGS II Kit is an updated version of the TGS I Kit.ApplicationsThe Template Generation System II Kit is designed for inserting an artificial Mu transposon into unknown target DNA to provide primer binding sites for:• Rapid sequencing of templates without primer walking or custom primers• Shotgun sequencing of large DNA clones directly without time-consuming fragmentation and subcloning into a library• Insertion of PCR priming sites into target DNA with an unknown sequence• Random insertional mutagenesis of cloned DNA; direct amplification, mapping or sequencing using primers at the insertion site• Sequencing with transposons vs. traditional sequencing• Special Applications:• BAC sequencing• Transposition reaction into linear target DNA

数据：

In vitro transposition reactionDuring a short 60 minute incubation, MuA Transposase binds to the Entranceposon and rapidly assembles into an active transposition complex. The complex attacks circular as well as linear target DNA and covalently joins the Entranceposon into the target DNA at random locations. The location of the transposon insertion in each individual clone can be mapped either by PCR or by sequencing.

技术参数

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Thermo Template Generation System II Kit,Template Generation System II Kit

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