Thermo Fisher PagI 内切酶 ER1281,PagI (BspHI、CciI、RcaI)(10 U/µL)/PagI 内切酶-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo Fisher PagI 内切酶 ER1281,PagI (BspHI、CciI、RcaI)(10 U/µL)/PagI 内切酶

2024-10-24

PagI (BspHI、CciI、RcaI)(10 U/µL)/PagI 内切酶

货号：ER1281,ER1282

规格：400 units,2000 units

价格：763,3047

产品类型：内切酶

品牌：Thermo Fisher

Thermo Scientific PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (isoschizomers: BspHI, CciI, RcaI). SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:Low salt, high glycerol (> 5%) concentrations, pH> 8.0 or a large excess of enzyme may result in star activity. PagI cleavage is impaired by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain such as GM2163. For methylation sensitivity, refer to product specifications.Thermo Scientific PagI (BspHI) 限制性内切酶可识别 T^CATGA 位点，于 37°C 下在O 缓冲液中的切割效果最佳（同裂酶：BspHI、CciI、RcaI）。有关该酶和其他限制性内切酶的酶活性、双重酶切条件和热灭活的表格，请参见限制性内切酶的反应条件。备注：另外提供用于快速 DNA 酶切的FastDigest 酶。Thermo Scientific 常规限制性核酸内切酶是高质量限制性内切酶的大量汇集物，经过优化以便在五缓冲液系统的一种缓冲液中发挥作用。此外还提供通用 Tango 缓冲液，便于进行双酶切。所有酶在推荐的缓冲液和反应条件下均表现出 100% 活性。为确保一致的性能，Thermo Scientific 限制性内切酶反应缓冲液包含预混合 BSA，其可增强多种酶的稳定性，并与 DNA 制剂中可能存在的污染物相结合。对 CpG 甲基化不敏感、对dam 甲基化敏感，对 dcm甲基化不敏感

优点：

- Superior quality—stringent quality control and industry leading manufacturing process- Convenient color-coded Five Buffer System- Includes universal Tango buffer for double-digestions- BSA premixed in reaction buffers- Wide selection of restriction endonuclease specificities- 卓越的质量—严格的质量控制和行业领先的生产工艺- 便利的颜色编码的五缓冲液系统- 包括用于双酶切的通用型 Tango 缓冲液- 在反应缓冲液中预混合 BSA- 多种特异性限制性核酸内切酶可供选择

数据：

技术参数

产品优点 1. 卓越的质量—严格的质量控制和行业领先的生产工艺 2. 便利的颜色编码的五缓冲液系统 3. 包括用于双酶切的通用型 Tango 缓冲液 4. 在反应缓冲液中预混合 BSA 5. 多种特异性限制性核酸内切酶可供选择

产品应用 •分子克隆 •限制位点作图 •基因分型 •Southern 印迹 •限制性片段长度多态性 (RFLP) •单核苷酸多态性（SNP）

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Thermo Fisher PagI 内切酶 ER1281,PagI (BspHI、CciI、RcaI)(10 U/µL)/PagI 内切酶

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