K1681 Thermo cDNA合成试剂盒,Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase/cDNA合成试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

K1681 Thermo cDNA合成试剂盒,Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase/cDNA合成试剂盒

2024-10-24

Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase/cDNA合成试剂盒

货号：K1681,K1682

规格：20 reactions,100 reactions

价格：1535,6134

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific dsDNase activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.For reverse transcription the kit uses Thermo Scientific Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived byin vitroevolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.Additional information about reaction components• Maxima H Minus Enzyme Mix containsMaxima H Minus Reverse TranscriptaseandRiboLock RNase Inhibitor. RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B and C at temperatures up to 55°C.•oligo(dT)18andrandom hexamerprimers are supplied with the kit. Random hexamer primers bind non-specifically and are used to synthesize cDNA from all RNAs in a total RNA population. The oligo(dT)18primer selectively anneals to the 3'-end of poly(A) RNA, synthesizing cDNA only from poly(A) tailed mRNA. Gene-specific primers may also be used with the kit to prime synthesis from a specified sequence.•10 mM dNTP Mixis a premixed aqueous solution of dATP, dTTP, dCTP, and dGTP.•Nuclease-free wateris provided for reaction set-up and dilution of sample DNA. The absence of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.

优点：

- Integrated genomic DNA removal step- Increased reaction temperatures—the first strand of cDNA can be synthesized within the 42 to 65°C temperature range- High yields of full-length first strand cDNA—with RNA templates up to 20 kb- Flexible priming—oligo(dT)18, random hexamer, or gene-specific primers

组成成分：

▪Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase contains Maxima H Minus Enzyme Mix, dsDNase, 10X dsDNase Buffer, Oligo(dT)18and Random hexamer primers, 5X RT Buffer, dNTP Mix, and nuclease-free water.

K1681 Thermo cDNA合成试剂盒,Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase/cDNA合成试剂盒

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