Thermo T4 DNA Ligase, LC (1 U/µL),T4 DNA Ligase, LC (1 U/µL)/T4 DNA连接酶,LC

2024-10-24

T4 DNA Ligase, LC (1 U/µL)/T4 DNA连接酶,LC

货号:EL0016

规格:2×500U 1U/μL

价格:851

产品类型:酶及抑制剂

品牌:Thermo Fisher

描述Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.T4 DNA Ligase requiresATPas a cofactor.Highlights• Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)• Fast—sticky-end ligation is completed in 10 minutes at room temperature• Supplied with PEG solution for efficient blunt-end ligationApplications• Cloning of restriction enzyme generated DNA fragments• Cloning of PCR products• Joining of double-stranded oligonucleotide linkers or adaptors to DNA• Site-directed mutagenesis• Amplified fragment length polymorphism (AFLP)• Ligase-mediated RNA detection (seeReference 3)• Nick repair in duplex DNA, RNA or DNA/RNA hybrids• Self-circularization of linear DNA.Includes• T4 DNA Ligase• 10X T4 DNA Ligase Buffer• 50% PEG SolutionNotes• Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with6X DNA Loading Dye & SDS Solutionat 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.• The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.• Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture using spin columns or chloroform extraction. The extracted DNA can be further precipitated with ethanol.

技术参数
© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://gibcohyclone.cn/thread-48344.htm
产品询价需求提交
返回
扫码添加客服微信,咨询产品报价