F553L 高保真PCR Thermo ,Phusion High-Fidelity PCR Kit/Phusion高保真PCR试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

F553L 高保真PCR Thermo ,Phusion High-Fidelity PCR Kit/Phusion高保真PCR试剂盒

2024-10-24

Phusion High-Fidelity PCR Kit/Phusion高保真PCR试剂盒

货号：F553L,F553S

规格：200 reactions,50 reactions

价格：2007,594

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that ofTaq, and 6-fold lower than that ofPfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

优点：

- High fidelity (52XTaq)- Fast PCR due to short extension times (15-30 s/kb)- Robust performance, minimal optimization needed- High yields of PCR products with minimal enzyme amounts- Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

数据：

图1.Superior yields of long PCR products

20kb fragment from λ DNA and 7.5 kb fragment from human genomic DNA was amplified with Phusion and proofreading DNA polymerase from other suppliers. Phusion and Phusion Green DNA Polymerases were the only enzymes capable of providing high amounts of desired PCR products. In contrast, other proofreading polymerases delivered zero or significantly lower yields.

图2.Phusion DNA Polymerase combines extreme fidelity with unparalleled speed and robustnessA random set of 16 clones from aThermus sp.genomic library was amplified from bacterial colonies. The results highlight the robustness and speed of Phusion DNA Polymerase. It was able to amplify 94% of the amplicons with lower enzyme amounts still producing superior yields. The success rate ofPfuDNA polymerase was only 56% and that ofTaqDNA polymerase 62% with significantly lower yields.

图3.Extreme fidelity for demanding PCRRelative fidelity values of different DNA polymerases. Phusion DNA Polymerases have extremely low error rates. The error rate, determined by a modified lacI-based method, is approximately 50-fold lower than that ofTaqDNA polymerase and 6-fold lower than that ofPfuDNA polymerase. Fidelity = 1/error rate.

图4.Less enzyme - superior yieldA 3.8kb fragment from human beta globin gene was amplified with three different DNA polymerases. Phusion DNA Polymerase was able to amplify the 3.8kb genomic fragment with a combined annealing and extension step of only 1minute, thus being significantly faster than the two other polymerases tested. A single unit of Phusion DNA Polymerase produced higher yields than 2.5 or 5 units of the Pfu DNA polymerases.

组成成分：

▪Phusion DNA Polymerase (2 U/µL)▪ 5X Phusion GC Buffer▪ 5X Phusion HF Buffer▪ dNTP mix (10 mM each)▪ MgCl2solution (50 mM)▪ Control template (λ DNA)▪ 1.3 kb primers (4 µM each)▪ 10 kb primers (4 µM each)▪ DNA size standard▪ DMSOStore at -20°C.

相关产品：

▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化

技术参数

产品应用 • High-fidelity PCR • Cloning • Template generation for sequencing • Amplification of difficult (GC-rich) templates • Long-range PCR (up to 20 kb) • Mutagenesis • High throughput PCR • Microarray Thermo Scientific Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control lambda DNA template and primers for 1.3 kb and 10 kb amplicons. The DNA template amount is sufficient for performing 20 control reactions in 50 µL volume or 50 control reactions in 20 µL volume.

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F553L 高保真PCR Thermo ,Phusion High-Fidelity PCR Kit/Phusion高保真PCR试剂盒

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