F547L PCR Thermo ,Phusion Blood Direct PCR Kit/Phusion血直接PCR试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

F547L PCR Thermo ,Phusion Blood Direct PCR Kit/Phusion血直接PCR试剂盒

2024-10-24

Phusion Blood Direct PCR Kit/Phusion血直接PCR试剂盒

货号：F547L,F547S

规格：500 reactions,100 reactions

价格：5112,1278

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion Blood Direct PCR Kit专为扩增全血中的DNA而研发，试剂盒中包含500 x 20 µL反应所需的全部试剂。本品能够免去PCR之前分离DNA的纯化步骤。经修饰的Phusion Hot Start II高保真DNA聚合酶对血液样本中存在的PCR抑制剂具有很高的耐受性，在优化条件下对含有40%全血的样本仍保有聚合酶活性。该DNA聚合酶在校正性能方面表现出卓越的精确性，这使得Phusion Blood Direct PCR Kit成为直接PCR以及如分子克隆一类需求更高应用的最佳选择。Phusion Blood Direct PCR试剂盒具有多种功能，广泛适用于分析多种物种，以及不同抗凝剂处理或储存条件下的血液样本。经测试的材料样例• 小鼠血液• 猪血• 猫血• 狗血• 牛血• 鸟类血液• 人血• 以肝素、柠檬酸盐或EDTA形式预存的血液样本• 储存于Whatman 903和FTA卡中的血液样本

优点：

- 无需进行费时而昂贵的DNA抽提- 简便的实验方案，最短的手动操作时间- 极短的PCR实验时间- 修饰过的Phusion热启动II高保真DNA聚合酶特异性好、产量高

数据：

图1.High yields from blood samples stored on various cards

Amplification of three DNA fragments from human blood collected on various filter papers. Piko Thermal Cycler and UTW reaction vessels were used in PCR. + and - denote control reactions.1 – 903 Card2 – FTA Gene Card3 – FTA Elute Card

图2.Robust amplification from a wide variety of speciesAmplification of a 237bp DNA fragment directly from whole blood of seven different vertebrates (5%blood in the reaction). + and - denote control reactions

图3.Superior performance even at very high blood concentrationsThe Phusion Blood Direct PCR Kit was compared to other kits designed for PCR directly from blood. A 588 bp genomic DNA fragment was amplified in the presence of increasing blood concentration in the reaction mixture. PCR was performed according to suppliers instructions using Piko Thermal Cycler and ultra-thin walled UTW reaction vessels. Total protocol times indicated at bottom. + and - denote control reactions.

组成成分：

▪200 µL Phusion Blood II DNA Polymerase▪ 5 x 1.25 mL 2X Phusion Blood PCR Buffer (includes dNTPs and MgCl2)▪ 40 µL Universal Control Primer Mix▪ 0.5 mL 50 mM EDTA▪ 1.5 mL 50 mM MgCl2▪ 0.5 mL DMSOStore at -20°C.

F547L PCR Thermo ,Phusion Blood Direct PCR Kit/Phusion血直接PCR试剂盒

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