2. 试剂
  3. F541 Thermo ,Phusion Site-Directed Mutagenesis Kit/位点导向突变试剂盒

F541 Thermo ,Phusion Site-Directed Mutagenesis Kit/位点导向突变试剂盒

2024-10-24
Phusion Site-Directed Mutagenesis Kit/位点导向突变试剂盒

货号：F541

规格：20 reactions

价格：1941

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase. The resulting plasmid can be then transformed into any competentE. colicells.The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.

优点：

- Robust and reliable exponential amplification method- No requirements, such as special vectors, restriction sites, or methylation status for the target plasmid- No need to destroy the starting template in a separate step-Phusion Hot Start II High Fidelity DNA Polymeraseminimizes unwanted secondary mutations- Amplification of large plasmids up to 10 kb- Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR- T4 DNA Ligase included in the kit; no purification steps before or after ligation- Compatible with all strains of competentE. colicells

数据：

图1.The Phusion Site-Directed Mutagenesis ProtocolFlow chart of the Phusion Site-Directed Mutagenesis Kit protocol. F=5’phosphorylated forward primer, R=5’phosphorylated reverse primer

相关产品：

▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化

技术参数
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