F534L DNA聚合酶,Phusion Green High-Fidelity DNA Polymerase (2 U/µL)/绿色高保真DNA聚合酶(2U/µL)-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

F534L DNA聚合酶,Phusion Green High-Fidelity DNA Polymerase (2 U/µL)/绿色高保真DNA聚合酶(2U/µL)

2024-10-24

Phusion Green High-Fidelity DNA Polymerase (2 U/µL)/绿色高保真DNA聚合酶(2U/µL)

货号：F534L,F534S

规格：500 units,100 units

价格：3074,783

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that ofTaq, and 6-fold lower than that ofPfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.The Phusion Green format is a combination of Phusion High-Fidelity DNA Polymerase with 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The density reagent and dyes do not interfere with excellent performance of Phusion DNA Polymerases and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

优点：

- High fidelity (52XTaq)- Fast PCR due to short extension times (15-30 s/kb)- Robust performance, minimal optimization needed- High yields of PCR products with minimal enzyme amounts- Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

数据：

图1.Superior yields of long PCR products

20kb fragment from λ DNA and 7.5 kb fragment from human genomic DNA was amplified with Phusion and proofreading DNA polymerase from other suppliers. Phusion and Phusion Green DNA Polymerases were the only enzymes capable of providing high amounts of desired PCR products. In contrast, other proofreading polymerases delivered zero or significantly lower yields.

图2.Robust amplification of DNA fragments regardless of GC contentFour DNA fragments of different GC content were amplified. Phusion Green DNA Polymerase produced all four amplicons with high yields. In contrast, competing high fidelity DNA polymerase was not able to efficiently produce GC-rich content.

组成成分：

▪Phusion DNA Polymerase, 2 U/µL▪ 5X Phusion Green HF Buffer▪ 5X Phusion Green GC Buffer▪ DMSO▪ 50 mM MgCl2Both Phusion Green HF and Phusion Green GC Buffer provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.

F534L DNA聚合酶,Phusion Green High-Fidelity DNA Polymerase (2 U/µL)/绿色高保真DNA聚合酶(2U/µL)

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