Klenow Fragment, LC (2 U/µL)

货号：EP0054

规格：300 units

价格：460

产品类型：酶及抑制剂

品牌：Thermo Fisher

描述Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.Highlights• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)• Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffersApplications• DNA blunting by fill-in 5'-overhangs• Random-primed DNA labeling• Labeling by fill-in 5'-overhangs of dsDNA• DNA sequencing by the Sanger method• Site-specific mutagenesis of DNA with synthetic oligonucleotides• Second strand synthesis of cDNA

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