T3 RNA Polymerase, HC (≥100 U/µL)/T3 RNA聚合酶, HC

货号：EP0103

规格：10000 units 200U/μL

价格：2037

产品类型：酶及抑制剂

品牌：Thermo Fisher

描述Thermo Scientific Bacteriophage T3 RNA polymerase is a DNA-dependent RNA polymerase with strict specificity for its respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter and is able to incorporate modified nucleotide.Highlights•Incorporates modified nucleotides(e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)Applications• Synthesis of unlabeled and labeled RNA that can be used:• For hybridization,in vitroRNA translation• As aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing• In studies of RNA secondary structure and RNA-protein interactions, RNA splicingNoteConsensus promoter sequence:T3:AATTAACCCTCACTAAAGGGAGAThe position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively.

技术参数

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