K0502,GeneJET Plasmid Miniprep Kit/GeneJET 质粒小提试剂盒

2024-10-24

GeneJET Plasmid Miniprep Kit/GeneJET 质粒小提试剂盒

货号:K0502,K0503

规格:50 preps,250 preps

价格:374,1493

产品类型:质粒

品牌:Thermo Fisher

Thermo Scientific GeneJET 质粒小提试剂盒采用独有的基于可靠硅膜技术离心柱。本试剂盒每次分离实验能够获得高达20 µg高拷贝质粒DNA。原理收获和裂解细菌培养物,之后通过离心收集裂解产物上清液,并加入硅胶柱内,通过较高的盐浓度条件选择性收集DNA分子。对吸附的DNA进行漂洗以清除污染物,之后将质粒DNA纯化产物洗脱至较小体积的洗脱缓冲液或水中。DNA纯化产物可直接应用于所有分子生物学操作中,包括快速和常规的限制性酶切、PCR、 体外转录、转化和自动测序等。

优点:

-高效—可获得高达20 µg的优质质粒DNA-快速—整个操作流程少于14分钟-便利—无需苯酚/氯仿抽提或酒精沉淀步骤-纯度高—可直接使用的DNA纯化产物

数据:

图1.Fast clone analysis
  • A 2.3kb PCR fragment was cloned into pUC19 vector. Plasmid DNA from overnight bacterial cultures of recombinant clones was purified using the GeneJET Plasmid Miniprep Kit and analyzed by double digestion with FastDigest EcoRI and FastDigest HindIII in 5minutes at 37°C.
  • M1, M2 –ZipRuler Express DNA Ladder Set
  • C – control pUC19 DNA digested with FastDigest EcoRI and HindIII
  • 1-6 – miniprep DNA from recombinant clones, double digested with FastDigest EcoRI and HindIII

图2.Overview of purification procedure

图3.High quality sequencing data of plasmid DNA purified with GeneJET Plasmid Miniprep KitPlasmid DNA was purified using the GeneJET Plasmid Miniprep Kit fromE. coliand sequenced with a T7 sequencing primer. The sequencing reaction was analyzed on an ABI® 3730 DNA Analyzer. More than 750 nucleotides can be read.

组成成分:

▪Resuspension Solution

▪Lysis Solution

▪Neutralization Solution*

▪Wash Solution (concentrated)

▪RNase A (10 mg/mL)

▪Elution Buffer (10 mM Tris-HCl, pH 8.5)

▪GeneJET Spin Columns

▪Collection Tubes (2 mL)

相关产品:

▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化

技术参数

产品应用 • 快速分离纯化适用于所有传统分子生物学实验的高纯度质粒DNA,这些后续实验包括: • 快速或常规限制性酶切 • 自动荧光或放射性测序 • PCR • 体外转录 • 转化

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