GeneJET Stabilized and Fresh Whole Blood RNA Kit

货号：K0871

规格：50 preps

价格：2993

产品类型：DNA提取

品牌：Thermo Fisher

描述Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).Highlights•Versatile—compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus™ Tubes•Convenient—specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h•Efficient—up to 4 µg of total RNA from 500 µL of healthy human blood•Pure—isolates intact total RNA free of any RT-qPCR inhibitorsApplicationsIsolated total RNA can be used in:• RT-PCR• RT-qPCR• Northern BlottingIncludes• Lysis Buffer (R2841)• Wash Buffer 1 (conc.)(R2851)• Wash Buffer 2 (conc.)(R2861)• DNase l (lyophilized)• DNase I Reconstitution Buffer• 2x DNase I Buffer• Manganese Chloride Solution• Water, nuclease-free• GeneJET RNA Purification Columns pre-assembled with Collection Tubes• Collection Tubes, 2 mL• Collection Tubes, 1.5 mLRelated ProductsLysis Buffer for GeneJET Blood RNA KitWash Buffer 1 for GeneJET Blood RNA Kit (concentrated)Wash Buffer 2 for GeneJET Blood RNA Kit (concentrated)Superior yields of isolated mRNATotal RNA was purified with the Thermo Scientific Kit and other vendors' kits using 250 µL of blood as starting material.All eluates were brought to the same volume.RNA was analysed by RT-qPCR using Thermo Scientific Verso 1-Step RT-qPCR SYBR Green Kit (AB-4104/A).

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