AID Antibody (39-2500) in IHC (P)Immunohistochemistry analysis of AID showing staining in the cytoplasm and nucleus of paraffin-embedded human lymph node tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a AID Mouse Monoclonal Antibody (Product # 39-2500) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. AID Antibody (39-2500)Cell research 2018 -Fig. 1 ROD1 physically interacts with AID via an ultraconserved loop region. a Diagram of the lamdaN/BoxB tethering assay and the mutation frequency observed in HEK293 cells. The C/G mutations to all C/G bases in BoxB region were calculated from 20 sequenced clones. b Silver staining of AID immunoprecipitates from lysates of either LPS-activated or naive splenic B cells. c ROD1 and AID interact with each other in LPS-activated B cells. The reciprocal co-IP was probed with anti-AID and anti-ROD1 antibodies. d Direct interaction between AID and ROD1 truncated proteins by GST pull-down assay. RRM RNA recognition motif, N-P N-terminal protein, C-P C-terminal protein, RBD3 RNA-binding domain 3, RBD4 RNA-binding domain 4. e The 3D interacting surface of AID (cyan) and ROD1 (green) modeled by PRISM. The key interacting amino acids are labeled in blue and indicated by arrowheads. f The residue composition and conservation of the loop region in ROD1. Amino acids from 504 to 513 were aligned across the animal kingdom. The mutated amino acids at each position are listed and marked by arrowheads. D.r. zebrafish, D.m. fly, X.I. frog, G.g. chicken, H.s. human, M.m. mouse AID Antibody (39-2500)Oncology letters 2017 -Figure 1. Representative histology, ISH and immunostaining images of EBV(+) GCLS, EBV(-) GC without LS and EBV(-) GCLS. H and E staining of (A) EBV(+) GCLS, (B) EBV(-) GC without LS and (C) EBV(-) GCLS. EBER1 ISH of (D) EBV(+) GCLS, (E) EBV(-) GC without LS and (F) EBV(-) GCLS. AID immunostaining of (G) EBV(+) GCLS, (H) EBV(-) GC without LS and (I) EBV(-) GCLS. NF-kappaB immunostaining of (J) EBV(+) GCLS, (K) EBV(-) GC without LS and (L) EBV(-) GCLS. PAX5 immunostaining of (M) EBV(+) GCLS, (N) EBV(-) GC without LS and (O) EBV(-) GCLS. c-Myb immunostaining of (P) EBV(+) GCLS, (Q) EBV(-) GC without LS and (R) EBV(-) GCLS. Magnification, x100. (G, I) Red stars indicate AID expression as the positive internal control observed in the lymphoid cells of the germinal center. ISH, in situ hybridization; EBV, Epstein-Barr virus; GCLS, GC with LS; GC, gastric carcinoma; LS, lymphoid stroma; H and E, hematoxylin and eosin; EBER1, EBV-encoded small RNA 1; AID, activation-induced cytidine deaminase; NF-kappaB, nuclear factor kappaB; PAX5, paired box 5; c-Myb, c-Myb proto-oncogene. |
QQ:1749072012