Bcl-2 Monoclonal Antibody (100/D5)/Bcl-2单克隆抗体（100 / D5）

货号：MA5-11757

规格：500 µL

价格：4880

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys)

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：100/D5

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.4 mg/mL	用法：	0.5-1 µg/test(Flow)；1:10-1:100(ICC)；1:10-1:100(IF)；1:50(IHC (P))；2 µg/mg protein lysate(IP)；1:50(WB)

产品详细信息MA5-11757 targets BCL-2 in FACS, ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human and mouse samples.The MA5-11757 immunogen is a synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.靶标信息BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

Bcl-2 Antibody (MA5-11757) in IFImmunofluorescent analysis of BCL-2 alpha (green) showing staining in the nucleus and cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a BCL-2 alpha monoclonal antibody (Product # MA5-11757) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x. Bcl-2 Antibody (MA5-11757) in IFImmunofluorescent analysis of BCL-2 alpha (green) showing staining in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a BCL-2 alpha monoclonal antibody (Product # MA5-11757) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x. Bcl-2 Antibody (MA5-11757) in IHCFormalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining of follicular cells and no staining of reactive cells in the germinal center.

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参考文献：

1 PeerJSunset Yellow and Allura Red modulate Bcl2 and COX2 expression levels and confer oxidative stress-mediated renal and hepatic toxicity in male rats.2 OncoTargets and therapyMelittin induces NSCLC apoptosis via inhibition of miR-183.3 CancersThe Potential Impact of Connexin 43 Expression on Bcl-2 Protein Level and Taxane Sensitivity in Head and Neck Cancers-In Vitro Studies.4 International journal of ophthalmologyParthenolide inhibits the proliferation and induces the apoptosis of human uveal melanoma cells.5 CancersSimvastatin Induces Apoptosis in Medulloblastoma Brain Tumor Cells via Mevalonate Cascade Prenylation Substrates.

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