p53 Monoclonal Antibody (BP53-12)/p53单克隆抗体（BP53-12）

货号：MA1-19055

规格：100 µg

价格：4880

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：Bacterially expressed full-length wild-type p53

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

克隆号：BP53-12

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	Assay Dependent(ELISA)；Assay Dependent(ICC)；Assay Dependent(IHC (P))；Assay Dependent(IP)；1-2 µg/mL(WB)

产品详细信息This antibody recognizes a defined epitope (aa 16-25) on human p53.靶标信息The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

p53 Antibody (MA1-19055) in WBA549 cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ON-TARGETplus TP53 siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading. p53 Antibody (MA1-19055)IP-MS enrichment of TP53 (LFQ intensity): TP53 was enriched 1381-fold from BT549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and TP53 antibody (Product # MA1-19055). STRING database was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. p53 Antibody (MA1-19055)Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. A549 cells were transfected with p53 siRNA and decrease in signal intensity was observed in western blot application using p53 Monoclonal Antibody (Product # MA1-19055).

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参考文献：

1 Oncology lettersAssociation between lysyl oxidase and fibrotic focus in relation with inflammation in breast cancer.2 Brain pathology (Zurich, Switzerland)CASP9 germline mutation in a family with multiple brain tumors.3 HistopathologyMorphology and genetics of pyloric gland adenomas in familial adenomatous polyposis.4 Journal of virologyThe Human Papillomavirus 16 E7 Oncoprotein Attenuates AKT Signaling To Promote Internal Ribosome Entry Site-Dependent Translation and Expression of c-MYC.5 PloS oneMultiple breast cancer cell-lines derived from a single tumor differ in their molecular characteristics and tumorigenic potential.

技术参数

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