Thermo,Pierce Glutathione Magnetic Agarose Beads/Pierce谷胱甘肽磁性琼脂糖珠-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo,Pierce Glutathione Magnetic Agarose Beads/Pierce谷胱甘肽磁性琼脂糖珠

2024-10-24

Pierce Glutathione Magnetic Agarose Beads/Pierce谷胱甘肽磁性琼脂糖珠

货号：78601,78602

规格：1 mL,5 mL

价格：2518,8279

产品类型：免疫印迹/组化

品牌：Thermo Fisher

Thermo Scientific™ Pierce™ Glutathione Magnetic Agarose Beads provide a fast, convenient method for purification of glutathione-S-transferase (GST) from a bacterial, yeast, or mammalian crude cell lysate. Pierce™ Glutathione Magnetic Agarose Beads are well suited for purifying GST fusion proteins from a soluble protein extract and are ideal for the purification of proteins expressed at low levels from diluted supernatants. The beads can be used in manual applications with a magnetic stand or automated applications with an instrument such as the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor. Automated instruments are especially useful for higher throughput purification and screening of purification conditions.The high-performance, magnetite, superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the KingFisher™ 96 and KingFisher™ Flex magnetic particle processors, but the beads also enable premium performance for simple benchtop purification applications using an appropriate magnetic stand.Glutathione S-transferase (GST) is a protein that is commonly added to the end of recombinant proteins to aid in their purification or detection. To make an affinity support, glutathione is immobilized through its central carbon, preserving the essential structure necessary for efficient GST binding. Addition of reduced glutathione in a physiologic buffer is sufficient to competitively displace and recover the GST fusion protein from the immobilized glutathione.

优点和特点：

• High-performance beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS applications• Stable affinity ligand—glutathione is covalently immobilized to particles, enabling leach-resistance and clean purification products• High capacity—binding capacity is sufficient for both routine and demanding purification procedures

数据：

High purity GST fusion protein purificationPierce Glutathione Magnetic Agarose provides high purity purification of recombinant GST-tagged proteins. Cell lysates (250 µL) containing GST-tagged Green Renialla Luciferase were diluted with an equal volume of binding buffer and purified using magnetic agarose beads (25 µL settled volume) following manufacturer's protocol. Proteins were purified according to the manufacturer’s instructions. Eluted fractions (2 µg total protein) were separated by SDS-PAGE and analyzed for percent purity. Lane 1: PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa (Cat No. 26619), lane 2: cell lysate, lane 4-11: 2 µg of eluted fractions.

Manual vs. automated purification methodsThermo Scientific™ Pierce™ Glutathione Magnetic Agarose Beads are suited for purifying GST fusion proteins by manual or automated methods. GST-tagged RalGDS and GreenRenillaluciferase were purified from cell lysates following a manual purification method using a magnetic stand and an automated purification method using the Thermo Scientific KingFisher™ Flex Magnetic Particle Processor.

High purification capacityThermo Scientific™ Pierce™ Glutathione Magnetic Agarose Beads provide high-capacity, high-yield purification of recombinant GST-tagged proteins. Cell lysates containing GST-tagged RalGDS (Guanine Nucleotide Dissociation Stimulator) or GST-GreenRenillaLuciferase were incubated with one of the following: Pierce Glutathione Magnetic Agarose Beads, Promega™ MagneGST™ Particles, or Sigma-Aldrich™ Glutathione High Capacity Magnetic Agarose Beads. Proteins were purified according to the manufacturer’s instructions. Lane 1: PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa (Cat. No. 26619); lane 2: cell lysate; lanes 4–11: purified GST-tagged protein.

技术参数

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Thermo,Pierce Glutathione Magnetic Agarose Beads/Pierce谷胱甘肽磁性琼脂糖珠

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