Human 13-Plex Human ProcartaPlex Panel 2, Platinum

货号：EPXP130-10100-901

规格：96 tests

价格：1

产品类型：多因子检测

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

样品体积:	Serum, Plasma: 25 µL
样品类型:	Serum, Plasma
Assay Sensitivity:	See product documentation
Bead Region:	See product documentation
Former Identifiers:	eBioscience
Multiplex Panel Name:	Human
Multiplex Size Plex No.:	13
Multiplex Panel Version:	2
Target Kit Named:	Human
System Type:	Luminex
Target Species Primary:	Human
Target List:	IL-12; GM-CSF; IFN gamma; IL-1 beta; IL-10; IL-13; IL-17A; IL-2; IL-4; IL-5; IL-6; IL-8 (CXCL8); TNF alpha
Target Species Named:	Human
Product Line:	ProcartaPlex
Label or Dye:	R-PE
Immunoassay Kit Format:	Panel
Target Species Validated:	Human
Detection Method:	Fluorescent
适用于（设备）:	Luminex Instruments
Immunoassay Kit Grade:	Preconfigured Panel

The Human Platinum 13-Plex ProcartaPlex Panel enables the study of immune function by analyzing 13 protein targets in a single well using Luminex xMAP technology. This panel assures high performance specifications (e.g., spike and dilution recovery results of 70-130%) by using matrix type specific diluents for plasma and serum that help overcome matrix effects. The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup.ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit ourProcartaPlex Immunoassays pagefor more information and available products.Target list [bead region]:GM-CSF [44], IFN gamma [43], IL-1 beta [18], IL-10 [28], IL-12p70 [34], IL-13 [35], IL-17A (CTLA-8) [36], IL-2 [19], IL-4 [20], IL-5 [21], IL-6 [25], IL-8 (CXCL8) [27], TNF alpha [45]About ProcartaPlex assays for the Luminex platformProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.Features include:• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitationProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

技术参数

组成成分 2 vials Platinum Human Standard Mix P12 vials Platinum Human Control P1 High2 vials Platinum Human Control P1 Low1 vial Platinum Human Panel 2 Bead Mix1 vial Platinum Human Panel 2 Detection Antibody Mix1 bottle Reading Buffer1 bottle Wash Buffer 10x1 bottle Streptavidin-PE1 bottle Assay Diluent for Plasma Samples1 bottle Assay Diluent for Serum SamplesPCR 8-Tube StripAdhesive FilmGreiner Microplate black 96 well

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