CD9 Monoclonal Antibody (eBioSN4 (SN4 C3-3A2)), eBioscience

货号：14-0098-82,14-0098-37,14-0098-95

规格：100 μg,2 mg,10 mg

市场价格：1334,11920,29802

产品类型：流式抗体

品牌：eBioscience

抗原：CD9

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：eBioSN4 (SN4 C3-3A2)

荧光染料：其它

类型:	人B细胞流抗	同型对照：	IgG1, kappa
浓度：	0.5 mg/mL	用法：	0.5 μg/test(Flow); Assay-Dependent(IHC(F)); Assay-Dependent(IP)

产品详细信息Description:The eBioSN4 monoclonal antibody reacts with human CD9. CD9 is a 24 kDa member of the tetraspanin family, whose members are characterized by the presence of 4 hydrophobic transmembrane domains. CD9 is expressed in platelets, eosinophils, basophils, pre-B cells, activated T cells and neural cell lines. Furthermore, CD9 expression has been associated with a malignant phenotype, including expression on 90% of non T cell acute lymphoblastic leukemia cells and on 50% of chronic lymphocytic and acute myeloblastic leukemias. In platelets, CD9 is expressed in alpha-granules and through association with the integrin alphaIIb/betaIII plays a role in platelet aggregation, as demonstrated by the use of anti-human CD9 antibodies. CD9 has also been shown to induce the aggregation of pre-B cell lines, and the adhesion and migration of pre-B cells and Schwann cells. Additionally, it has been demonstrated that CD9 is able to provide a co-stimulatory signal for T cells independently of CD28, in the absence of antigen-presenting cells. Binding of the eBioSN4 monoclonal antibody partially cross-blocks binding of another anti-human CD9 monoclonal antibody, MM2/57.Applications Reported: This eBioSN4 (SN4 C3-3A2) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.Applications Tested: This eBioSN4 (SN4 C3-3A2) antibody has been tested by flow cytometric analysis of normal human platelets. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD9 Antibody (14-0098-82) in FlowStaining of normal human peripheral blood cells with 0.25 µg of Mouse IgG11 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD9 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.

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参考文献：

1.Nature communicationsCirculating exosomes suppress the induction of regulatory T cells via let-7i in multiple sclerosis."14-0098 was used in Western Blotting to show that exosomal let-7i regulates MS pathogenesis by blocking the IGF1R/TGFBR1 pathway.2.CellStereotypic Immune System Development in Newborn Children."14-0098 was used in Flow cytometry/Cell sorting to study stereotypic immune system development in newborn children."3.Nature communicationsHuman islets contain four distinct subtypes of β cells."Published figure using CD9 monoclonal antibody (Product # 14-0098-82) in Flow Cytometry"4.PloS oneMicrovesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo."Published figure using CD9 monoclonal antibody (Product # 14-0098-82) in Flow Cytometry"

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