CD275 (B7-H2) Monoclonal Antibody (MIH12), PE, eBioscience

货号：12-5889-42

规格：100 Tests

市场价格：2365

产品类型：流式抗体

品牌：eBioscience

抗原：CD275

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：MIH12

荧光染料：PE

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test（Flow）

产品详细信息Description: The MIH12 monoclonal antibody reacts with human B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. Human B7RP-1 is expressed by activated monocytes/macrophages. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.Applications Reported: The MIH12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MIH12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood monocyte-derived dendritic cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.仅用于科研。不用于诊断过程。

数据

CD275 (B7-H2) Antibody (12-5889-42) in FlowStaining of normal human peripheral blood monocyte-derived dendritic cells with Mouse IgG1 K Isotype Control PE (open histogram) (Product # 12-4714-81) or Anti-Human CD275 (B7-H2) PE (filled histogram). Total viable cells were used for analysis.

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献：

1.Frontiers in immunologyTargeting the Human T-Cell Inducible COStimulator Molecule with a Monoclonal Antibody Prevents Graft-vs-Host Disease and Preserves Graft vs Leukemia in a Xenograft Murine Model."12-5889 was used in Flow cytometry/Cell sorting to determine the role of monoclonal antibody to human ICOS in costimulatory signalling to human T cells."AuthorsBurlion A,Brunel S,Petit NY,Olive D,Marodon G2. Nature communicationsBiallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity."12-5889 was used in Flow cytometry/Cell sorting to study the effect of a biallelic mutation in the gene encoding NF-κB-inducing kinase on immune cell populations."AuthorsWillmann KL,Klaver S,Doğu F,Santos-Valente E,Garncarz W,Bilic I,Mace E,Salzer E,Conde CD,Sic H,Májek P,Banerjee PP,Vladimer GI,Haskoloğlu S,Bolkent MG,Küpesiz A,Condino-Neto A,Colinge J,Superti-Furga G,Pickl WF,van Zelm MC,Eibel H,Orange JS,Ikincioğulları A,Boztuğ KYear2014SpeciesHuman3. Journal of immunology (Baltimore, Md. : 1950)CagA-dependent downregulation of B7-H2 expression on gastric mucosa and inhibition of Th17 responses during Helicobacter pylori infection."12-5889 was used in Flow cytometry/Cell sorting to investigate the impact of H.pylori CagA on the modulation of the expression of the T cell costimulator B7-H2 by gastric epithelial cells."AuthorsLina TT,Pinchuk IV,House J,Yamaoka Y,Graham DY,Beswick EJ,Reyes VEYear2013SpeciesHuman4. Arthritis and rheumatismNovel role of plasmacytoid dendritic cells in humans: induction of interleukin-10-producing Treg cells by plasmacytoid dendritic cells in patients with rheumatoid arthritis responding to therapy."12-5889 was used in Flow cytometry/Cell sorting to explore the contribution of dendritic cells to the development of regulatory T cells in a human autoimmune disease setting."AuthorsKavousanaki M,Makrigiannakis A,Boumpas D,Verginis PYear2010SpeciesHuman5.Scandinavian journal of immunologyExpression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues.AuthorsYoungnak-Piboonratanakit P,Tsushima F,Otsuki N,Igarashi H,Omura K,Azuma M

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-48801.htm