CD275 (B7-H2) Monoclonal Antibody (MIH12), PE-eFluor 610, eBioscience

货号：61-5889-42,61-5889-41

规格：100 Tests,25 Tests

市场价格：3019,1199

产品类型：流式抗体

品牌：eBioscience

抗原：CD275

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：MIH12

荧光染料：PE-eFluor 610

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor 610, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test（Flow）

产品详细信息Description: The MIH12 monoclonal antibody reacts with human B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. Human B7RP-1 is expressed by activated monocytes/macrophages. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.Applications Reported: This MIH12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MIH12 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood monocyte-derived dendritic cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser靶标信息Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.仅用于科研。不用于诊断过程。

数据

CD275 (B7-H2) Antibody (61-5889-42) in FlowNormal human peripheral blood monocyte-derived dendritic cells were stained with CD11c Monoclonal Antibody, FITC (Product # 11-0116-42) and Mouse IgG1 kappa Isotype Control, PE-eFluor 610 (Product # 61-4714-82) (left) or CD275 (B7H2) Monoclonal Antibody, PE-eFluor 610 (right). Total viable cells were used for analysis.

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技术参数

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