CD314 (NKG2D) Monoclonal Antibody (1D11), FITC, eBioscience

货号：11-5878-42

规格：100 tests

市场价格：2630

产品类型：流式抗体

品牌：eBioscience

抗原：CD314

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：1D11

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test (Flow）

产品详细信息Description:The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.Applications Reported: The 1D11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1D11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular Cterminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD314 (NKG2D) Antibody (11-5878-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD8a eFluor® 450 (Product # 48-0086-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD314 (NKG2D) FITC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Cancer biology & therapyResveratrol sensitized leukemia stem cell-like KG-1a cells to cytokine-induced killer cells-mediated cytolysis through NKG2D ligands and TRAIL receptors."11-5878 was used in Flow cytometry/Cell sorting to investigate the therapeutic potential of resveratrol to sensitise human promyeloblastic leukemia KG-1a cells to cytolysis."2.Bioscience reportsmiR-20a regulates sensitivity of colorectal cancer cells to NK cells by targeting MICA."Published figure using CD314 (NKG2D) monoclonal antibody (Product # 11-5878-42) in Flow Cytometry"3.Oncology lettersNatural killer cells inhibit pulmonary metastasis of hepatocellular carcinoma in nude mice."11-5878 was used in Flow cytometry/Cell sorting to assess whether NK cells activated by IL-2 can inhibit the metastasis of hepatocellular carcinoma in vitro and in vivo."

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