CD16 Monoclonal Antibody (eBioCB16 (CB16)),

货号：14-0168-82

规格：898.5

市场价格：1283

产品类型：流式抗体

品牌：eBioscience

抗原：CD16

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：eBioCB16 (CB16)

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.Applications Reported: This eBioCB16 (CB16) antibody has been reported for use in flow cytometric analysis. Binding of the eBioCD16 antibody is not blocked by binding of the MEM-154 human CD16 monoclonal antibody.Applications Tested: This eBioCB16 (CB16) antibody has been tested by flow cytometric analysis of normal normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.

数据

CD16 Antibody (14-0168-82) in FlowStaining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD16 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte (left) and granulocyte (right) gates were used for analysis.

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参考文献

1. Pediatric transplantationChanges in natural killer cell subsets in pediatric liver transplant recipients.2. Nature communicationsLECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.3. Stem cell reportsRetinoic acid regulates hematopoietic development from human pluripotent stem cells.4. PloS oneHigher Frequency of NK and CD4+ T-Cells in Mucosa and Potent Cytotoxic Response in HIV Controllers.5. Stem cell reportsRetinoic acid regulates hematopoietic development from human pluripotent stem cells.

技术参数

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