CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), PE, eBioscience

货号：12-1038-42,12-1038-41

规格：100 Tests,25 Tests

市场价格：2476,1029

产品类型：流式抗体

品牌：eBioscience

抗原：CD103

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号： B-Ly7

荧光染料：PE

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test（Flow）

产品详细信息Description: The B-Ly7 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta 7. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen, E-cadherin, binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.Applications Reported: B-Ly7 has been reported for use in flow cytometric analysis.Applications Tested: This B-Ly7 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is part of the integrin family which are heterodimeric integral membrane proteinscomposed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.仅用于科研。不用于诊断过程。

数据

CD103 (Integrin alpha E) Antibody (12-1038-42) in FlowStaining of PHA-stimulated normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD103 (Integrin alpha E) PE (purple histogram). Total viable cells were used for analysis.

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参考文献：

1. Frontiers in immunologyEvidence for Resident Memory T Cells in Rasmussen Encephalitis."12-1038 was used in Flow cytometry/Cell sorting to demonstrate that a cellular immune response to an as yet unidentified antigen(s) occurs at an early stage of Rasmussen encephalitis."AuthorsOwens GC,Chang JW,Huynh MN,Chirwa T,Vinters HV,Mathern GWYear2016SpeciesHuman

2. Genome biologyDistinct epigenetic features of tumor-reactive CD8+ T cells in colorectal cancer patients revealed by genome-wide DNA methylation analysis."12-1038 was used in Flow cytometry/Cell sorting to show the CD8+ T cells of colorectal cancer patients can are influenced by distinct DNA methylation patterns."AuthorsYang R,Cheng S,Luo N,Gao R,Yu K,Kang B,Wang L,Zhang Q,Fang Q,Zhang L,Li C,He A,Hu X,Peng J,Ren X,Zhang ZYear2019SpeciesHumanDilution1:100

3. PloS oneCD1c-Expression by Monocytes - Implications for the Use of Commercial CD1c+ Dendritic Cell Isolation Kits."12-1038 was used in Flow cytometry/Cell sorting to demonstrate that a widely-used commercial CD1c+ dendritic cell isolation kit isolates two phenotypically and functionally different CD1c+ cell populations."AuthorsSchrøder M,Melum GR,Landsverk OJ,Bujko A,Yaqub S,Gran E,Aamodt H,Bækkevold ES,Jahnsen FL,Richter L

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