CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), eBioscience

货号：14-1038-82,14-1038-37,14-1038-95

规格：100 ug,2 mg,10 mg

市场价格：1283,11562,28906

产品类型：流式抗体

品牌：eBioscience

抗原：CD103

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：B-Ly7

荧光染料：Purified

类型:	一抗	同型对照：	IgG1, kappa
浓度：	0.5 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The B-Ly7 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta 7. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen, E-cadherin, binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.Applications Reported: B-Ly7 has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: The B-Ly7 antibody has been tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is part of the integrin family which are heterodimeric integral membrane proteinscomposed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.仅用于科研。不用于诊断过程。

数据

CD103 (Integrin alpha E) Antibody (14-1038-82) in FlowStaining of 3-day PHA-stimulated normal human peripheral blood cells with 0.25 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD103 (Integrin alpha E) Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.

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参考文献：

1. Journal of immunology (Baltimore, Md. : 1950)Tissue Distribution of Memory T and B Cells in Rhesus Monkeys following Influenza A Infection."14-1038 was used in Flow cytometry/Cell sorting to identify a subset of influenza-specific lung memory T-cells characterized as tissue-resident memory T-cells in rhesus monkeys."AuthorsPichyangkul S,Yongvanitchit K,Limsalakpetch A,Kum-Arb U,Im-Erbsin R,Boonnak K,Thitithayanont A,Jongkaewwattana A,Wiboon-ut S,Mongkolsirichaikul D,Mahanonda R,Spring M,Chuang I,Mason CJ,Saunders DLYear2015SpeciesRhesus monkey 2. Journal of immunology (Baltimore, Md. : 1950)Rate of AIDS progression is associated with gastrointestinal dysfunction in simian immunodeficiency virus-infected pigtail macaques."14-1038 was used in Flow cytometry/Cell sorting to determine whether pre-existing GI-associated mucosal damage affects the progression of AIDS following HIV infection."AuthorsCanary LA,Vinton CL,Morcock DR,Pierce JB,Estes JD,Brenchley JM,Klatt NRYear2013SpeciesNon-human primate 3. BloodDNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues."14-1038 was used in Flow cytometry/Cell sorting to investigate the presence of unique markers in human dendritic cells, showing that DNGR-1 is a specific marker of mouse and human dendritic cells in lymphoid and nonlymphoid tissues."AuthorsPoulin LF,Reyal Y,Uronen-Hansson H,Schraml BU,Sancho D,Murphy KM,Håkansson UK,Moita LF,Agace WW,Bonnet D,Reis e Sousa CYear2012SpeciesHuman 4. The Journal of experimental medicineCharacterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells."14-1038 was used in Flow cytometry/Cell sorting to investigate a population of human DCs that expresses DNGR-1 and high levels of BDCA3."AuthorsPoulin LF,Salio M,Griessinger E,Anjos-Afonso F,Craciun L,Chen JL,Keller AM,Joffre O,Zelenay S,Nye E,Le Moine A,Faure F,Donckier V,Sancho D,Cerundolo V,Bonnet D,Reis e Sousa C

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