CD335 (NKp46) Monoclonal Antibody (9E2), Super Bright 600,

货号：63-3359-41,63-3359-42

规格：25 Tests,100 Tests

市场价格：1771,3432

产品类型：流式抗体

品牌：eBioscience

抗原：CD335 (NKp46)

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：9E2

荧光染料：Super Bright 600

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright 600
浓度：	5 µL/Test	用法：	5 µL (0.03 µg)/test(Flow)

产品详细信息Description: This 9E2 monoclonal antibody reacts with human CD335, which is also known as NKp46. Expressed on NK cells, CD335 is a member of the natural cytotoxicity receptor family that also includes NKp30 and NKp44. This type I transmembrane glycoprotein is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor and virus-infected (e.g., influenza) cells. CD335 also interacts with pancreatic beta cells during the development of type Idiabetes.The 9E2 antibody has been reported to induce cytotoxicity.Applications Reported: This 9E2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 9E2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human periheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 600 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

数据

CD335 (NKp46) Antibody (63-3359-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) FITC (Product # 11-0566-42) and Mouse IgG1 K Isotype Control Super Bright 600 (Product # 63-4714-82) (left) or Anti-Human CD335 (NKp46) Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis.

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