CD21/CD35 Monoclonal Antibody (eBio4E3 (4E3)), eFluor 450, eBioscience

货号：48-0212-82

规格：100 µg

市场价格：2892

产品类型：流式抗体

品牌：eBioscience

抗原：CD21/CD35

物种：小鼠

宿主：大鼠

抗体亚型：其它

克隆号：eBio4E3 (4E3)

荧光染料：eFluor 450

类型:	功能抗体	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), eFluor 450, eBioscience
浓度：	0.2 mg/mL	用法：	0.5 μg/test(Flow)

产品详细信息Description:

The monoclonal antibody eBio4E3 reacts with an eptiope shared by mouse CD21 (CR2) and CD35 (CR1). CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, which result in cell-surface proteins of 145 and 190 kDa, respectively. CD21 and CD35 are expressed by mature B cells, but not on thymocytes, peripheral T cells, erythrocytes or platelets. Furthermore, there is little evidence which demonstrates their expression on macrophages. CD21 is a receptor for complement component C3d and Epstein-Barr virus (EBV), and in association with CD19 and CD81, CD21 also participates in B-cell activation through the B cell receptor. Cr2 deficient mice display impaired inflammatory and humoral immune responses in vivo. The anti-mouse CD21/35 monoclonal antibody clones eBio4E3 and eBio8D9 do not cross-block each other suggesting that they bind to different epitopes.Applications Reported: This eBio4E3 (4E3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio4E3 (4E3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD21/CD35 Antibody (48-0212-82) in FlowStaining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a kappa Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.25 µg of Anti-Mouse CD21/CD35 eFluor® 450 (right). Total viable cells were used for analysis.

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参考文献：

1.Biochemical and biophysical research communicationsPostnatal lethality and abnormal development of foregut and spleen in Ndrg4 mutant mice."Published figure using CD21/CD35 monoclonal antibody (Product # 48-0212-82) in Immunofluorescence"2.Frontiers in immunologyMALT1 Proteolytic Activity Suppresses Autoimmunity in a T Cell Intrinsic Manner."48-0212 was used in Flow cytometry/Cell sorting to establish that suppression of autoimmune responses is due to the T-cell intrinsic role of MALT1 proteolytic activity."3.Journal of immunology (Baltimore, Md. : 1950)Age-Associated B Cells Express a Diverse Repertoire of VHand Vκ Genes with Somatic Hypermutation."48-0212 was used in Flow cytometry/Cell sorting to show that quiescent ABCs are Ag-experienced cells that accumulate during T cell-dependent responses to diverse Ags during the life of an individual."

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