CD86 (B7-2) Monoclonal Antibody (GL1), APC, eBioscience
货号:17-0862-81,17-0862-82
规格:50 μg,100 μg
市场价格:1674,2788
产品类型:流式抗体
品牌:eBioscience
抗原:CD86
物种:其它
宿主:大鼠
抗体亚型:IgG2a, kappa
克隆号:GL1
荧光染料:APC
类型: | 流式抗体 | 同型对照: | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience |
浓度: | 0.2 mg/mL | 用法: | 0.06 μg/test(Flow) |
产品详细信息Description:
The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.Applications Reported: The GL1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The GL1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is theligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
数据 |
CD86 (B7-2) Antibody (17-0862-82) in FlowStaining of unstimulated (left) or 3-day LPS-stimulated (right) C57BL/6 splenocytes with 0.03 µg of Rat IgG2a kappa Isotype Control APC (Product # 17-4321-81) (open histogram) or 0.03 µg of Anti-Mouse CD86 (B7-2) APC (filled histogram). Total viable cells were used for analysis. |
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参考文献: |
1.Nature communicationsLin-CCR2+hematopoietic stem and progenitor cells overcome resistance to PD-1 blockade."17-0862 was used in Flow cytometry/Cell sorting to elucidate a novel role for CCR2+ haematopoietic stem cells in overcoming brain tumour resistance to PD-1 checkpoint blockade and adoptive cellular therapy in multiple invasive brain tumour models."2.Journal of immunotoxicologyEvaluation of the irritancy and hypersensitivity potential following topical application of didecyldimethylammonium chloride."17-0862 was used in Flow cytometry/Cell sorting to investigate the irritancy and hypersensivity potential of didecyldimethylammonium chloride (DDAC)."3.Frontiers in immunologyMicroRNA-7 Deficiency Ameliorates the Pathologies of Acute Lung Injury through Elevating KLF4."17-0862 was used in Flow cytometry/Cell sorting to study the role of microRNA-7 in acute lung injury, allowing the development of new therapeutic strategies against inflammatory lung disease." |
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