IgD Monoclonal Antibody (11-26c (11-26)), Super Bright 702, eBioscience

货号：67-5993-82

规格：100 µg

市场价格：3244

产品类型：流式抗体

品牌：eBioscience

抗原： IgD

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号： 11-26c (11-26)

荧光染料：Super Bright 702

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 702, eBioscience
浓度：	0.2 mg/mL	用法：	0.125 µg/test（Flow）

产品详细信息Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.\tApplications Reported: This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes.This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 702 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.仅用于科研。不用于诊断过程。

数据

IgD Antibody (67-5993-82) in FlowBALB/c mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, APC (Product # 17-0452-82) and 0.06 µg of Rat IgG2a kappa Isotype Control, Super Bright 702 (Product # 67-4321-82) (left) or 0.06 µg of IgD Monoclonal Antibody, Super Bright 702 (right). Cells in the lymphocyte gate were used for analysis.

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-48912.htm