Mouse IgM Monoclonal Antibody (eB121-15F9), Super Bright 436,

货号：62-5890-82

规格：100 µg

市场价格：2997

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eB121-15F9

荧光染料：Super Bright 436

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 436
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.Applications Reported: This eB121-15F9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eB121-15F9 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

Mouse IgM Antibody (62-5890-82) in FlowC57BL/6 mouse bone marrow cells were stained with CD45R Monoclonal Antibody, APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4321-82) (left) or 0.25 µg of IgM Monoclonal Antibody, Super Bright 436 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

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