CD80 (B7-1) Monoclonal Antibody (16-10A1), PE, eBioscience

货号：12-0801-85,12-0801-83,12-0801-82,12-0801-81

规格：500 µg,200 µg,100 µg,50 µg

市场价格：5057,2892,2000,1038

产品类型：流式抗体

品牌：eBioscience

抗原：CD80

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：16-10A1

荧光染料：PE

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience
浓度：	0.2 mg/mL	用法：	0.06 µg/test（Flow）

产品详细信息Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: The 16-10A1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD80 (B7-1) Antibody (12-0801-85) in FlowMouse splenocytes were unstimulated (left) or stimulated for 2 days with F (ab')2 Anti-Mouse IgM, u chain specific Functional Grade Purified (Product # 16-5092-85) and Anti-Mouse CD40 Functional Grade Purified (Product # 16-0401-82) (right). Cells were then stained with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.03 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (blue histogram) or 0.03 µg of Anti-Mouse CD80 (B7-1) PE (purple histogram). Cell in the CD45R (B220) positive gate were used for analysis.

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参考文献：

1. OncoimmunologyAngiotensin converting enzyme inhibitors and angiotensin II receptor antagonist attenuate tumor growth via polarization of neutrophils toward an antitumor phenotype."12-0801 was used in Flow cytometry/Cell sorting to investigate how modulation of Ang II pathway attenuates tumour growth via polarisation of neutrophils to an antitumoural phenotype."AuthorsShrestha S,Noh JM,Kim SY,Ham HY,Kim YJ,Yun YJ,Kim MJ,Kwon MS,Song DK,Hong CWYear2021SpeciesMouse 2. OncoimmunologyT cell inhibitory mechanisms in a model of aggressive Non-Hodgkin's Lymphoma.AuthorsHilmenyuk T,Ruckstuhl CA,Hayoz M,Berchtold C,Nuoffer JM,Solanki S,Keun HC,Beavis PA,Riether C,Ochsenbein AF 3. Journal of immunology (Baltimore, Md. : 1950)Restricted microbiota and absence of cognate TCR antigen leads to an unbalanced generation of Th17 cells."12-0801 was used in Flow cytometry/Cell sorting to demonstrate that a complex microbiota and cognate antigen are required to generate a properly regulated set of Foxp3+RORγt+ T cells and Th17 cells."AuthorsLochner M,Bérard M,Sawa S,Hauer S,Gaboriau-Routhiau V,Fernandez TD,Snel J,Bousso P,Cerf-Bensussan N,Eberl GYear2011SpeciesMouse 4. ImmunologyAnti-tumour synergy of cytotoxic chemotherapy and anti-CD40 plus CpG-ODN immunotherapy through repolarization of tumour-associated macrophages."12-0801 was used in Flow cytometry/Cell sorting to show that chemotherapy and anti-CD40 + CpG-ODN IT synergise in the induction of anti-tumour effects which are associated with the phenotypic repolarisation of tumour-associated Mφ."AuthorsBuhtoiarov IN,Sondel PM,Wigginton JM,Buhtoiarova TN,Yanke EM,Mahvi DA,Rakhmilevich ALYear2011SpeciesMouse 5. Investigative ophthalmology & visual scienceInhibition of B-cell activation by retinal pigment epithelium."12-0801 was used in Flow cytometry/Cell sorting to determine whether retinal pigment epithelial (RPE) cells can inhibit B-cell activation in vitro, showing that B-cell proliferation was suppressed."AuthorsSugita S,Horie S,Yamada Y,Mochizuki M

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